Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy

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dc.contributor.author Covolan, Luciene [UNIFESP]
dc.contributor.author Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
dc.date.accessioned 2015-06-14T13:36:19Z
dc.date.available 2015-06-14T13:36:19Z
dc.date.issued 2006-07-01
dc.identifier http://dx.doi.org/10.1590/S0100-879X2006000700010
dc.identifier.citation Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 39, n. 7, p. 915-924, 2006.
dc.identifier.issn 0100-879X
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/3146
dc.description.abstract Pilocarpine-induced (320 mg/kg, ip) status epilepticus (SE) in adult (2-3 months) male Wistar rats results in extensive neuronal damage in limbic structures. Here we investigated whether the induction of a second SE (N = 6) would generate damage and cell loss similar to that seen after a first SE (N = 9). Counts of silver-stained (indicative of cell damage) cells, using the Gallyas argyrophil III method, revealed a markedly lower neuronal injury in animals submitted to re-induction of SE compared to rats exposed to a single episode of pilocarpine-induced SE. This effect could be explained as follows: 1) the first SE removes the vulnerable cells, leaving behind resistant cells that are not affected by the second SE; 2) the first SE confers increased resistance to the remaining cells, analogous to the process of ischemic tolerance. Counting of Nissl-stained cells was performed to differentiate between these alternative mechanisms. Our data indicate that different neuronal populations react differently to SE induction. For some brain areas most, if not all, of the vulnerable cells are lost after an initial insult leaving only relatively resistant cells and little space for further damage or cell loss. For some other brain areas, in contrast, our data support the hypothesis that surviving cells might be modified by the initial insult which would confer a sort of excitotoxic tolerance. As a consequence of both mechanisms, subsequent insults after an initial insult result in very little damage regardless of their intensity. en
dc.format.extent 915-924
dc.language.iso eng
dc.publisher Associação Brasileira de Divulgação Científica
dc.relation.ispartof Brazilian Journal of Medical and Biological Research
dc.rights Acesso aberto
dc.subject Cell injury en
dc.subject Status epilepticus en
dc.subject Epilepsy en
dc.subject Pilocarpine en
dc.subject Spontaneous seizures en
dc.title Assessment of the progressive nature of cell damage in the pilocarpine model of epilepsy en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia
dc.description.affiliationUnifesp UNIFESP, Depto. de Fisiologia
dc.identifier.file S0100-879X2006000700010.pdf
dc.identifier.scielo S0100-879X2006000700010
dc.identifier.doi 10.1590/S0100-879X2006000700010
dc.description.source SciELO
dc.identifier.wos WOS:000239624200010



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