Human immature dental pulp stem cells' contribution to developing mouse embryos: production of human/mouse preterm chimaeras

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dc.contributor.author Siqueira da Fonseca, S. A.
dc.contributor.author Abdelmassih, S.
dc.contributor.author Cintra Lavagnolli, T. de Mello [UNIFESP]
dc.contributor.author Serafim, R. C.
dc.contributor.author Clemente Santos, E. J.
dc.contributor.author Mendes, C. Mota
dc.contributor.author Pereira, V. de Souza
dc.contributor.author Ambrosio, C. E.
dc.contributor.author Miglino, M. A.
dc.contributor.author Visintin, J. A.
dc.contributor.author Abdelmassih, R.
dc.contributor.author Kerkis, A.
dc.contributor.author Kerkis, I.
dc.date.accessioned 2016-01-24T13:52:25Z
dc.date.available 2016-01-24T13:52:25Z
dc.date.issued 2009-04-01
dc.identifier http://dx.doi.org/10.1111/j.1365-2184.2008.00578.x
dc.identifier.citation Cell Proliferation. Malden: Wiley-Blackwell, v. 42, n. 2, p. 132-140, 2009.
dc.identifier.issn 0960-7722
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31439
dc.description.abstract In this study, we aimed at determining whether human immature dental pulp stem cells (hIDPSC) would be able to contribute to different cell types in mouse blastocysts without damaging them. Also, we analysed whether these blastocysts would progress further into embryogenesis when implanted to the uterus of foster mice, and develop human/mouse chimaera with retention of hIDPSC derivates and their differentiation.hIDPSC and mouse blastocysts were used in this study. Fluorescence staining of hIDPSC and injection into mouse blastocysts, was performed. Histology, immunohistochemistry, fluorescence in situ hybridization and confocal microscopy were carried out.hIDPSC showed biological compatibility with the mouse host environment and could survive, proliferate and contribute to the inner cell mass as well as to the trophoblast cell layer after introduction into early mouse embryos (n = 28), which achieved the hatching stage following 24 and 48 h in culture. When transferred to foster mice (n = 5), these blastocysts with hIDPSC (n = 57) yielded embryos (n = 3) and foetuses (n = 6); demonstrating presence of human cells in various organs, such as brain, liver, intestine and hearts, of the human/mouse chimaeras. We verified whether hIDPSC would also be able to differentiate into specific cell types in the mouse environment. Contribution of hIDPSC in at least two types of tissues (muscles and epithelial), was confirmed. We showed that hIDPSC survived, proliferated and differentiated in mouse developing blastocysts and were capable of producing human/mouse chimaeras. en
dc.description.sponsorship Roger Abdelmassih Human Reproduction Clinic and Research Center
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship São Paulo, Brazil
dc.format.extent 132-140
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Cell Proliferation
dc.rights Acesso restrito
dc.title Human immature dental pulp stem cells' contribution to developing mouse embryos: production of human/mouse preterm chimaeras en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Roger Abdelmassih Human Reprod Clin
dc.contributor.institution Res Ctr
dc.contributor.institution Butantan Inst
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Roger Abdelmassih Human Reprod Clin, Stem Cell Lab, BR-04131000 São Paulo, Brazil
dc.description.affiliation Res Ctr, BR-04131000 São Paulo, Brazil
dc.description.affiliation Butantan Inst, Genet Lab, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Vet Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Morphol & Genet, São Paulo, Brazil
dc.identifier.doi 10.1111/j.1365-2184.2008.00578.x
dc.description.source Web of Science
dc.identifier.wos WOS:000264185000002



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