Human immature dental pulp stem cells' contribution to developing mouse embryos: production of human/mouse preterm chimaeras

Human immature dental pulp stem cells' contribution to developing mouse embryos: production of human/mouse preterm chimaeras

Author Siqueira da Fonseca, S. A. Google Scholar
Abdelmassih, S. Google Scholar
Cintra Lavagnolli, T. de Mello Autor UNIFESP Google Scholar
Serafim, R. C. Google Scholar
Clemente Santos, E. J. Google Scholar
Mendes, C. Mota Google Scholar
Pereira, V. de Souza Google Scholar
Ambrosio, C. E. Google Scholar
Miglino, M. A. Google Scholar
Visintin, J. A. Google Scholar
Abdelmassih, R. Google Scholar
Kerkis, A. Google Scholar
Kerkis, I. Google Scholar
Institution Roger Abdelmassih Human Reprod Clin
Res Ctr
Butantan Inst
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract In this study, we aimed at determining whether human immature dental pulp stem cells (hIDPSC) would be able to contribute to different cell types in mouse blastocysts without damaging them. Also, we analysed whether these blastocysts would progress further into embryogenesis when implanted to the uterus of foster mice, and develop human/mouse chimaera with retention of hIDPSC derivates and their differentiation.hIDPSC and mouse blastocysts were used in this study. Fluorescence staining of hIDPSC and injection into mouse blastocysts, was performed. Histology, immunohistochemistry, fluorescence in situ hybridization and confocal microscopy were carried out.hIDPSC showed biological compatibility with the mouse host environment and could survive, proliferate and contribute to the inner cell mass as well as to the trophoblast cell layer after introduction into early mouse embryos (n = 28), which achieved the hatching stage following 24 and 48 h in culture. When transferred to foster mice (n = 5), these blastocysts with hIDPSC (n = 57) yielded embryos (n = 3) and foetuses (n = 6); demonstrating presence of human cells in various organs, such as brain, liver, intestine and hearts, of the human/mouse chimaeras. We verified whether hIDPSC would also be able to differentiate into specific cell types in the mouse environment. Contribution of hIDPSC in at least two types of tissues (muscles and epithelial), was confirmed. We showed that hIDPSC survived, proliferated and differentiated in mouse developing blastocysts and were capable of producing human/mouse chimaeras.
Language English
Sponsor Roger Abdelmassih Human Reproduction Clinic and Research Center
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
São Paulo, Brazil
Date 2009-04-01
Published in Cell Proliferation. Malden: Wiley-Blackwell, v. 42, n. 2, p. 132-140, 2009.
ISSN 0960-7722 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 132-140
Origin http://dx.doi.org/10.1111/j.1365-2184.2008.00578.x
Access rights Closed access
Type Article
Web of Science ID WOS:000264185000002
URI http://repositorio.unifesp.br/handle/11600/31439

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