Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion

Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion

Author Silva, Claudio V. da Autor UNIFESP Google Scholar
Kawashita, Silvia Y. Autor UNIFESP Google Scholar
Probst, Christian M. Google Scholar
Dallagiovanna, Bruno Google Scholar
Cruz, Mario C. Autor UNIFESP Google Scholar
Silva, Erika A. da Autor UNIFESP Google Scholar
Souto-Padron, Thais C. B. S. Google Scholar
Krieger, Marco A. Google Scholar
Goldenberg, Samuel Google Scholar
Briones, Marcelo R. S. Autor UNIFESP Google Scholar
Andrews, Norma W. Google Scholar
Mortara, Renato A. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fiocruz MS
Universidade Federal do Rio de Janeiro (UFRJ)
Yale Univ
Abstract Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. the recombinant form (P21-His(6)) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His(6) inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His(6) augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization. (C) 2009 Elsevier Masson SAS. All rights reserved.
Keywords Trypanosoma cruzi
Cell invasion
Metacyclic trypomastigote
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 03/12962-0
Date 2009-04-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 11, n. 5, p. 563-570, 2009.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 563-570
Access rights Closed access
Type Article
Web of Science ID WOS:000267839100004

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