Inflammatory events induced by brown spider venom and its recombinant dermonecrotic toxin: A pharmacological investigation

Inflammatory events induced by brown spider venom and its recombinant dermonecrotic toxin: A pharmacological investigation

Author Paludo, Katia Sabrina Autor UNIFESP Google Scholar
Petris Biscaia, Stellee Marcela Google Scholar
Chaim, Olga Meiri Autor UNIFESP Google Scholar
Otuki, Michel Fleith Google Scholar
Naliwaiko, Katya Google Scholar
Dombrowski, Patricia Andreia Google Scholar
Franco, Celia Regina Cavichiolo Autor UNIFESP Google Scholar
Veiga, Silvio Sanches Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Abstract Accidents involving Brown spider (Loxosceles sp.) venom produce a massive inflammatory response in injured region. This venom has a complex mixture of different toxins, and the dermonecrotic toxin is the major contributor to toxic effects. the ability of Loxosceles intermedia venom and a recombinant isoform of dermonecrotic toxin to induce edema and increase in vascular permeability was investigated. These toxins were injected into hind paws and caused a marked dose and time-dependent edema and increase in vascular permeability in mice. Furthermore, the enzymatic activity of venom toxins may be primal for these effects. A mutated recombinant isoform of dermonecrotic toxin, that has only residual enzymatic activity, was not able to induce these inflammatory events. Besides the previous heating of toxins markedly reduced the paw edema and vascular permeability showing that thermolabile constituents can trigger these effects. in addition, the ability of these venom toxins to evoke inflammatory events was partially reduced in compound 48/80-pretreated animals, suggesting that mast cells may be involved in these responses. Pretreating mice with histamine (prometazine and cetirizine) and serotonin (methysergide) receptor antagonists significantly attenuated toxins induced edema and vascular permeability. Moreover, HPLC analysis of whole venom showed the presence of histamine sufficient to induce inflammatory responses. in conclusion, these inflammatory events may result from the activation of mast cells, which in turn release bioamines and may be related to intrinsic histamine content of venom. (C) 2008 Published by Elsevier Inc.
Keywords Histamine
Loxosceles intermedia
Mast cells
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundacao Araucaria-PR
Secretaria de Estado de Ciencia, Tecnologia e Ensino Superior do Parana, Brazil
Date 2009-04-01
Published in Comparative Biochemistry and Physiology C-toxicology & Pharmacology. New York: Elsevier B.V., v. 149, n. 3, p. 323-333, 2009.
ISSN 1532-0456 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 323-333
Access rights Closed access
Type Article
Web of Science ID WOS:000264699400009

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