Unique behavior of Trypanosoma dionisii interacting with mammalian cells: Invasion, intracellular growth, and nuclear localization

Unique behavior of Trypanosoma dionisii interacting with mammalian cells: Invasion, intracellular growth, and nuclear localization

Author Castro Oliveira, Miriam Pires de Autor UNIFESP Google Scholar
Cortez, Mauro Autor UNIFESP Google Scholar
Maeda, Fernando Yukio Autor UNIFESP Google Scholar
Fernandes, Maria Cecilia Autor UNIFESP Google Scholar
Haapalainen, Edna Freymuller Autor UNIFESP Google Scholar
Yoshida, Nobuko Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The phylogenetic proximity between Trypanosoma cruzi and Trypanosoma (Schizotrypanum) dionisii suggests that these parasites might explore similar strategies to complete their life cycles. T, cruzi is the etiological agent of the life threatening Chagas' disease, whereas T dionisii is a bat trypanosome and probably not capable of infecting humans. Here we sought to compare mammalian cell invasion and intracellular traffic of both trypanosomes and determine the differences and similarities in this process. the results presented demonstrate that T dionisii is highly infective in vitro, particularly when the infection process occurs without serum and that the invasion is similarly affected by agents known to interfere with T. cruzi invasion process. Our results indicate that the formation of lysosomal-enriched compartments is part of a cell-invasion mechanism retained by related trypanosomatids, and that residence and further escape from a lysosomal compartment may be a common requisite for successful infection. During intracellular growth, parasites share a few epitopes with T. cruzi amastigotes and trypomastigotes. Unexpectedly, in heavily infected cells, amastigotes and trypomastigotes were found inside the host cell nucleus. These findings suggest that T. dionisii although sharing some features in host cell invasion with T. cruzi, has unique behaviors that deserve to be further explored. (c) 2009 Elsevier B.V. All rights reserved.
Keywords Trypanosoma dionisii
Trypanosoma cruzi
Trypomastigotes
Cell invasion
Nuclear invasion
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 05/56472-1
Date 2009-04-01
Published in Acta Tropica. Amsterdam: Elsevier B.V., v. 110, n. 1, p. 65-74, 2009.
ISSN 0001-706X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 65-74
Origin http://dx.doi.org/10.1016/j.actatropica.2009.01.008
Access rights Closed access
Type Article
Web of Science ID WOS:000264947500011
URI http://repositorio.unifesp.br/handle/11600/31412

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