Paracoccidioides brasiliensis Vaccine Formulations Based on the gp43-Derived P10 Sequence and the Salmonella enterica FliC Flagellin

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dc.contributor.author Braga, Catarina J. M.
dc.contributor.author Rittner, Glauce M. G.
dc.contributor.author Munoz Henao, Julian E.
dc.contributor.author Teixeira, Aline F.
dc.contributor.author Massis, Liliana M.
dc.contributor.author Sbrogio-Almeida, Maria E.
dc.contributor.author Taborda, Carlos Pelleschi [UNIFESP]
dc.contributor.author Travassos, Luiz R. [UNIFESP]
dc.contributor.author Ferreira, Luis C. S.
dc.date.accessioned 2016-01-24T13:52:23Z
dc.date.available 2016-01-24T13:52:23Z
dc.date.issued 2009-04-01
dc.identifier http://dx.doi.org/10.1128/IAI.01470-08
dc.identifier.citation Infection and Immunity. Washington: Amer Soc Microbiology, v. 77, n. 4, p. 1700-1707, 2009.
dc.identifier.issn 0019-9567
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31401
dc.description.abstract Paracoccidioidomycosis (PCM) is a systemic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis. Anti-PCM vaccine formulations based on the secreted fungal cell wall protein (gp43) or the derived P10 sequence containing a CD4(+) T-cell-specific epitope have shown promising results. in the present study, we evaluated new anti-PCM vaccine formulations based on the intranasal administration of P. brasiliensis gp43 or the P10 peptide in combination with the Salmonella enterica FliC flagellin, an innate immunity agonist binding specifically to the Toll-like receptor 5, in a murine model. BALB/c mice immunized with gp43 developed high-specific-serum immunoglobulin G1 responses and enhanced interleukin-4 (IL-4) and IL-10 levels. On the other hand, mice immunized with recombinant purified flagellins genetically fused with P10 at the central hypervariable domain, either flanked or not by two lysine residues, or the synthetic P10 peptide admixed with purified FliC elicited a prevailing Th1-type immune response based on lung cell-secreted type 1 cytokines. Mice immunized with gp43 and FliC and intratracheally challenged with P. brasiliensis yeast cells had increased fungal proliferation and lung tissue damage. in contrast, mice immunized with the chimeric flagellins and particularly those immunized with P10 admixed with FliC reduced P. brasiliensis growth and lung damage. Altogether, these results indicate that S. enterica FliC flagellin modulates the immune response to P. brasiliensis P10 antigen and represents a promising alternative for the generation of anti-PCM vaccines. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 1700-1707
dc.language.iso eng
dc.publisher Amer Soc Microbiology
dc.relation.ispartof Infection and Immunity
dc.rights Acesso aberto
dc.title Paracoccidioides brasiliensis Vaccine Formulations Based on the gp43-Derived P10 Sequence and the Salmonella enterica FliC Flagellin en
dc.type Artigo
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Inst Butantan
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ São Paulo, Dept Microbiol, ICB, BR-05008000 São Paulo, Brazil
dc.description.affiliation Inst Butantan, Div Desenvolvimento Tecnol & Prod, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil
dc.identifier.file WOS000264191500045.pdf
dc.identifier.doi 10.1128/IAI.01470-08
dc.description.source Web of Science
dc.identifier.wos WOS:000264191500045



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