Isoflavone regulates Vascular Endothelial Growth Factor Expression in urinary tract of castrated rats

Isoflavone regulates Vascular Endothelial Growth Factor Expression in urinary tract of castrated rats

Author Sampaio, Maria D. D. Autor UNIFESP Google Scholar
Jarmy-Di Bella, Zsuzsanna I. K. Autor UNIFESP Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Santos, Elaine T. Autor UNIFESP Google Scholar
Nogueira-de-Souza, Naiara Corrêa Autor UNIFESP Google Scholar
Zucchi, Eliana V. M. Autor UNIFESP Google Scholar
Simbes, Manuel de J. Autor UNIFESP Google Scholar
Girao, Manoel J. B. C. Autor UNIFESP Google Scholar
Sartori, Marair G. F. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: the purpose of this Study was to investigate Vascular Endothelial Growth Factor Expression (VEGF) gene regulation by isoflavone in urinary tract tissues of castrated adult rats.Design: Forty-five adult rats, 90 days old, weighting 200g were used, receiving a soy-free ration. the animals were castrated for drug administration for 30 days (125 mu g genisteine/g body weight/day) and sacrificed, divided into three groups: Group I-control: Group II-started isoflavone administration on the 5th day after castration; Group III-starred isoflavone administration on the 28th day after castration. RNA was isolated from each bladder and urethra. Determination of VEGF gene regulated by isoflavone was obtained using a semiquantitative RT-PCR and immunohistochemistry of total RNA isolated from bladder and urethra.Results: Our results demonstrate that isoflavone was able to upregulate mRNA level of the VEGF gene in the lower urinary tract of rats in Group II, where isoflavone administration was started at an early phase of estrogen deprivation, while in Group III, where isoflavone administration was started in the late phase of hypoestrogenism, did not show alteration of bladder and urethra VEGF gene expression, compared to placebo, maintaining the same level of the castrated rats without treatment.Conclusions: the data indicate that VEGF expression in rats is also regulated by isoflavone in early phase of hypoestrogenism. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
Keywords Isoflavone
Genistein
VEGF
Lower urinary tract
Language English
Date 2009-03-20
Published in Maturitas. Clare: Elsevier B.V., v. 62, n. 3, p. 317-320, 2009.
ISSN 0378-5122 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 317-320
Origin http://dx.doi.org/10.1016/j.maturitas.2009.01.003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000265523500017
URI http://repositorio.unifesp.br/handle/11600/31387

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