Interaction of polysaccharide-protein complex from Agaricus blazei with Langmuir and Langmuir-Blodgett films of phospholipids

Interaction of polysaccharide-protein complex from Agaricus blazei with Langmuir and Langmuir-Blodgett films of phospholipids

Autor Schmidt, Thais F. Google Scholar
Pavinatto, Felippe J. Google Scholar
Caseli, Luciano Autor UNIFESP Google Scholar
Gonzaga, Maria L. C. Google Scholar
Soares, Sandra A. Google Scholar
Ricardo, Nagila M. P. S. Google Scholar
Oliveira, Osvaldo N. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Univ Fed Ceara
Resumo The use of natural substances in health applications may be hampered by the difficulties in establishing the mechanisms of action, especially at molecular-level. the protein-polysaccharide complex extracted from the mushroom Agaricus blazei Murill, referred to as CAb, has been considered for treating various diseases with probable interaction with cell membranes. in this study, we investigate the interaction between CAb and a cell membrane model represented by a Langmuir monolayer of dimyristoyl phosphatidic acid (DMPA). CAb affects the structural properties of DMPA monolayers causing expansion and increasing compressibility. in addition, interaction with DMPA polar heads led to neutralization of the electrical double layer, yielding a zero surface potential at large areas per molecule. CAb remained at the interface even at high surface pressures, which allowed transfer of Langmuir-Blodgett (LB) films onto solid supports with the CAb-DMPA mixture. the mass transferred, according to quartz crystal microbalance (QCM) measurements, increased linearly with the number of deposited layers. With UV-vis absorption, fluorescence and FTIR spectroscopies, we confirmed that the LB films contain polysaccharides, proteins and DMPA. Therefore, the CAb biological action must be attributed not only to polysaccharides but also to proteins in the complex. (C) 2008 Elsevier Inc. All rights reserved.
Assunto Langmuir films
Polysaccharide-protein complexes
Mushroom Agaricus blazei Murill
Langmuir-Blodgett films
Cell membrane
DMPA
Air-water interface
Deposition
Compressibility
In-plane elasticity
Idioma Inglês
Financiador FAPESR IMMP
FINED
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Rede Biomat (Brazil)
Data 2009-02-01
Publicado em Journal of Colloid and Interface Science. San Diego: Academic Press Inc Elsevier Science, v. 330, n. 1, p. 84-89, 2009.
ISSN 0021-9797 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 84-89
Fonte http://dx.doi.org/10.1016/j.jcis.2008.10.024
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000262229700012
URI http://repositorio.unifesp.br/handle/11600/31296

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