No mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide

No mutations found in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline-1-oxide

Author Minicucci, Eliana Maria Google Scholar
Silva, Glenda Nicioli da Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Favero Salvadori, Daisy Maria Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. the present study aimed to investigate mutations in exon 2 of gene p16CDKN2A during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO) using direct DNA-sequencing method. A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide (4NQO) in drinking water for 4, 12, and 20 weeks at 50 ppm dose. Ten animals were used as negative control. No histopathological changes in tongue epithelia were observed among controls or in the group treated for 4 weeks with 4NQO. Following 12-week treatment, hyperplasia and epithelial dysplasia were found in mild and moderate forms. At 20 weeks, the tongue presented moderate and/or severe oral dysplasia and squamous cell carcinoma, with squamous cell carcinoma in the majority of animals. No mutations were found in any experimental period evaluated that corresponded to normal oral mucosa, hyperplasia, dysplasia and squamous cell carcinomas. Taken together, our results suggest that p16CDKN2A mutations in exon 2 are not involved in the multistep tongue carcinogenesis of Wistar rats induced by 4NQO.
Keywords p16
Rat tongue mucosa
Oral squamous cell carcinoma
4-Nitroquinoline 1-oxide
Direct DNA-sequencing
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 05/59156-3
FAPESP: 07/01228-4
Date 2009-02-01
Published in Journal of Molecular Histology. Dordrecht: Springer, v. 40, n. 1, p. 71-76, 2009.
ISSN 1567-2379 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 71-76
Origin http://dx.doi.org/10.1007/s10735-009-9215-4
Access rights Closed access
Type Article
Web of Science ID WOS:000264485300008
URI http://repositorio.unifesp.br/handle/11600/31282

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