Avaliação eletrofisiológica in vitro de drogas antiepilépticas em fatias hipocampais humanas provenientes de pacientes portadores de epilepsia do lobo temporal refratária ao tratamento medicamentoso

Avaliação eletrofisiológica in vitro de drogas antiepilépticas em fatias hipocampais humanas provenientes de pacientes portadores de epilepsia do lobo temporal refratária ao tratamento medicamentoso

Título alternativo In vitro electrophysiological assessment of antiepileptic drugs in human hippocampal slices originating from patients with refractory temporal lobe epilepsy
Autor Silva, André César da Autor UNIFESP Google Scholar
Yacubian, Elza Márcia Targas Autor UNIFESP Google Scholar
Sakamoto, Américo Ceiki Autor UNIFESP Google Scholar
Centeno, Ricardo Silva Autor UNIFESP Google Scholar
Ueda, Erika Sayuri Autor UNIFESP Google Scholar
Antônio, Leandro Leite Autor UNIFESP Google Scholar
Faria, Leonardo Coutinho Autor UNIFESP Google Scholar
Priel, Margareth Rose Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The absence of a satisfactory response to antiepileptic drug (AED) therapy, is an unresolved problem in a significant number of epileptic patients. Mechanisms of intractability are not well understood but may include a combination of poor penetration of AED across a functionally altered blood-brain barrier owing to increased expression of multiple drug resistance transporters. Therefore, the aim of this work was to assess the in vitro efficacy of antiepileptic drugs through human hippocampal slices originating from patients with refractory temporal lobe epilepsy submitted to corticoamygdalohippocampectomy. Slices was prepared from a 1 cm³ block of the hippocampus body 30 min after resection. Briefly, hippocampal slices of 400 µM thickness was cut coronally. Extracellular field potentials was recorded from the st. Granulosum of the dentate gyrus. The antiepileptic drugs added in the bath were Carbamazepine, Topiramate and Phenytoin. The phenytoin was effective reducing the hyperexcitability (polispikes) in 60% of the experiments (n = 5). On the other hand, the carbamazepine promoted a decrease in evoked epileptiform activity in 37,5% of the cases (n = 8). The application of topiramate in the bath reduced in 30% the number of polispikes (n = 10). Our results showed that the phenytoin application resulted in a significant reduction in neuronal excitability, however, the carbamazepine and topiramate were not able to control of the hiperexcitability, suggesting that local neuronal alterations, as well changes in blood brain barrier, could be responsible for such behaviors.
Palavra-chave antiepileptic drugs
in vitro electrophysiology
drug resistance
drogas antiepilépticas
electrofisiologia in vitro
drogas resistentes
Idioma Português
Data de publicação 2006-06-01
Publicado em Journal of Epilepsy and Clinical Neurophysiology. Liga Brasileira de Epilepsia (LBE), v. 12, n. 2, p. 73-74, 2006.
ISSN 1676-2649 (Sherpa/Romeo)
Publicador Liga Brasileira de Epilepsia (LBE)
Extensão 73-74
Fonte http://dx.doi.org/10.1590/S1676-26492006000300005
Direito de acesso Acesso aberto Open Access
Tipo Artigo
SciELO S1676-26492006000300005 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/3127

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