ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion

ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion

Author Silva, Claudio Vieira da Autor UNIFESP Google Scholar
Silva, Erika Alves da Autor UNIFESP Google Scholar
Cruz, Mario Costa Autor UNIFESP Google Scholar
Chavrier, Philippe Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Inst Curie
Universidade Federal de Uberlândia (UFU)
Abstract Toxoplasma gondii infects a variety of different cell types in a range of different hosts. Host cell invasion by T. gondii occurs by active penetration of the host cell, a process previously described as independent of host actin polymerization. Also, the parasitophorous vacuole has been shown to resist fusion with endocytic and exocytic pathways of the host cell. ADP-ribosylation factor-6 (ARF6) belongs to the ARE family of small GTP-binding proteins. ARF6 regulates membrane trafficking and actin cytoskeleton rearrangements at the plasma membrane. Here, we have observed that ARF6 is recruited to the parasitophorous vacuole of tachyzoites of T. gondii RH strain and it also plays an important role in the parasite cell invasion with activation of PI3-kinase and recruitment of PIP(2) and PIP(3) to the parasitophorous vacuole of invading parasites. Moreover, it was verified that maintenance of host cell actin cytoskeleton integrity is important to parasite invasion. (C) 2008 Elsevier Inc. All rights reserved.
Keywords Toxoplasma gondii
Host cell invasion
Parasitophorous vacuole
ARF6
PI3-kinase
Actin cytoskeleton
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 07/54300-4
Date 2009-01-16
Published in Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc Elsevier Science, v. 378, n. 3, p. 656-661, 2009.
ISSN 0006-291X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 656-661
Origin http://dx.doi.org/10.1016/j.bbrc.2008.11.108
Access rights Closed access
Type Article
Web of Science ID WOS:000262343200064
URI http://repositorio.unifesp.br/handle/11600/31251

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account