hTERT methylation and expression in gastric cancer

hTERT methylation and expression in gastric cancer

Author Gigek, Carolina Oliveira Google Scholar
Leal, Mariana Ferreira Google Scholar
Oliveira Silva, Patricia Natalia Google Scholar
Frias Lisboa, Luara Carolina Google Scholar
Lima, Eleonidas Moura Google Scholar
Calcagno, Danielle Queiroz Google Scholar
Assumpcao, Paulo Pimentel Google Scholar
Burbano, Rommel Rodriguez Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Abstract Gastric cancer is the second most prevalent cause of cancer death worldwide. DNA methylation is a common event in gastric carcinogenesis. hTERT seems to be the rate-limiting determinant of telomerase activation, which is responsible for stability and life span. hTERT hypermethylation has been associated with telomerase expression. in the present study, we investigated the promoter methylation status and hTERT protein expression in gastric cancer and normal mucosa samples. One hundred and nine gastric cancer and 53 normal mucosa samples were investigated through methylation-specific PCR. Immunohistochemistry was analysed using peroxidase in 55 gastric cancer and 18 normal gastric mucosa samples. This is the first study evaluating hTERT methylation status in gastric carcinogenesis. We did not observe hTERT protein expression in normal gastric mucosa. Moreover, hTERT expression was observed in 80% of tumours and was associated with gastric cancer (p < 0.0001). Partial methylation was the most frequent pattern in gastric samples, even in normal mucosa. the frequency of specimens presenting hypermethylation was significantly higher in tumours than in normal mucosa samples (p = 0.0002), although the presence of hypermethylated promoter was not associated with a higher frequency of hTERT expression. A low correlation between hTERT protein expression and methylation was verified in gastric cancer samples. There was a clear difference in the frequency of hTERT expression and methylation within tumoral and non-tumoral tissues. Methylation status and telomerase expression may be useful for the diagnosis of gastric cancer and may have an impact on the anti-telomerase strategy for cancer therapy.
Keywords Gastric cancer
protein expression
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2009-01-01
Published in Biomarkers. Abingdon: Taylor & Francis Ltd, v. 14, n. 8, p. 630-636, 2009.
ISSN 1354-750X (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 630-636
Access rights Closed access
Type Article
Web of Science ID WOS:000272717400011

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