Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine

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dc.contributor.author Valera, Elvis Terci
dc.contributor.author Abdalla de Freitas Cortez, Maria Angelica
dc.contributor.author Paula Queiroz, Rosane Gomes de
dc.contributor.author Oliveira, Fabio Morato de
dc.contributor.author Brassesco, Maria Sol
dc.contributor.author Jabado, Nada
dc.contributor.author Faury, Damien
dc.contributor.author Bobola, Michael S.
dc.contributor.author Machado, Helio Rubens
dc.contributor.author Scrideli, Carlos Alberto
dc.contributor.author Tone, Luiz Gonzaga
dc.date.accessioned 2016-01-24T13:52:02Z
dc.date.available 2016-01-24T13:52:02Z
dc.date.issued 2009-01-01
dc.identifier http://dx.doi.org/10.1007/s00381-008-0740-3
dc.identifier.citation Childs Nervous System. New York: Springer, v. 25, n. 1, p. 39-45, 2009.
dc.identifier.issn 0256-7040
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31152
dc.description.abstract Resistance to drug is a major cause of treatment failure in pediatric brain cancer. the multidrug resistance (MDR) phenotype can be mediated by the superfamily of adenosine triphosphate-binding cassette (ABC) transporters. the dynamics of expression of the MDR genes after exposure to chemotherapy, especially the comparison between pediatric brain tumors of different histology, is poorly described.To compare the expression profiles of the multidrug resistance genes ABCB1, ABCC1, and ABCG2 in different neuroepithelial pediatric brain tumor cell lines prior and following short-term culture with vinblastine.Immortalized lineages from pilocytic astrocytoma (R286), anaplasic astrocytoma (UW467), glioblastoma (SF188), and medulloblastoma (UW3) were exposed to vinblastine sulphate at different schedules (10 and 60 nM for 24 and 72 h). Relative amounts of mRNA expression were analyzed by real-time quantitative polymerase chain reaction. Protein expression was assessed by immunohistochemistry for ABCB1, ABCC1, and ABCG2.mRNA expression of ABCB1 increased together with augmenting concentration and time of exposure to vinblastine for R286, UW467, and UW3 cell lines. Interestingly, ABCB1 levels of expression diminished in SF188. Following chemotherapy, mRNA expression of ABCC1 decreased in all cell lines other than glioblastoma. ABCG2 expression was influenced by vinblastine only for UW3. the mRNA levels showed consistent association to protein expression in the selected sets of cell lines analyzed.The pediatric glioblastoma cell line SF188 shows different pattern of expression of multidrug resistance genes when exposed to vinblastine. These preliminary findings may be useful in determining novel strategies of treatment for neuroepithelial pediatric brain tumors. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 39-45
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Childs Nervous System
dc.rights Acesso restrito
dc.subject Cancer en
dc.subject Children en
dc.subject Brain tumor en
dc.subject Cell lines en
dc.subject Drug resistance en
dc.subject Chemotherapy en
dc.subject Genetics en
dc.title Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution McGill Univ
dc.contributor.institution Childrens Hosp
dc.contributor.institution Reg Med Ctr
dc.description.affiliation Univ São Paulo, Div Pediat Oncol, Dept Pediat, Fac Med Ribeirao Preto, BR-14048900 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Dept Genet, Fac Med Ribeirao Preto, BR-14048900 São Paulo, Brazil
dc.description.affiliation McGill Univ, Dept Pediat, Div Hemtooncol, Montreal, PQ H3A 2T5, Canada
dc.description.affiliation Childrens Hosp, Div Pediat Oncol, Seattle, WA USA
dc.description.affiliation Univ São Paulo, Div Neurosurg, Dept Surg & Anat, Fac Med Ribeirao Preto, BR-14048900 São Paulo, Brazil
dc.description.affiliation Reg Med Ctr, Seattle, WA USA
dc.description.sponsorshipID FAPESP: 2007/04065-9
dc.identifier.doi 10.1007/s00381-008-0740-3
dc.description.source Web of Science
dc.identifier.wos WOS:000261374800007



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