Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine

Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine

Author Valera, Elvis Terci Google Scholar
Abdalla de Freitas Cortez, Maria Angelica Google Scholar
Paula Queiroz, Rosane Gomes de Google Scholar
Oliveira, Fabio Morato de Google Scholar
Brassesco, Maria Sol Google Scholar
Jabado, Nada Google Scholar
Faury, Damien Google Scholar
Bobola, Michael S. Google Scholar
Machado, Helio Rubens Google Scholar
Scrideli, Carlos Alberto Google Scholar
Tone, Luiz Gonzaga Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
McGill Univ
Childrens Hosp
Reg Med Ctr
Abstract Resistance to drug is a major cause of treatment failure in pediatric brain cancer. the multidrug resistance (MDR) phenotype can be mediated by the superfamily of adenosine triphosphate-binding cassette (ABC) transporters. the dynamics of expression of the MDR genes after exposure to chemotherapy, especially the comparison between pediatric brain tumors of different histology, is poorly described.To compare the expression profiles of the multidrug resistance genes ABCB1, ABCC1, and ABCG2 in different neuroepithelial pediatric brain tumor cell lines prior and following short-term culture with vinblastine.Immortalized lineages from pilocytic astrocytoma (R286), anaplasic astrocytoma (UW467), glioblastoma (SF188), and medulloblastoma (UW3) were exposed to vinblastine sulphate at different schedules (10 and 60 nM for 24 and 72 h). Relative amounts of mRNA expression were analyzed by real-time quantitative polymerase chain reaction. Protein expression was assessed by immunohistochemistry for ABCB1, ABCC1, and ABCG2.mRNA expression of ABCB1 increased together with augmenting concentration and time of exposure to vinblastine for R286, UW467, and UW3 cell lines. Interestingly, ABCB1 levels of expression diminished in SF188. Following chemotherapy, mRNA expression of ABCC1 decreased in all cell lines other than glioblastoma. ABCG2 expression was influenced by vinblastine only for UW3. the mRNA levels showed consistent association to protein expression in the selected sets of cell lines analyzed.The pediatric glioblastoma cell line SF188 shows different pattern of expression of multidrug resistance genes when exposed to vinblastine. These preliminary findings may be useful in determining novel strategies of treatment for neuroepithelial pediatric brain tumors.
Keywords Cancer
Brain tumor
Cell lines
Drug resistance
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2007/04065-9
Date 2009-01-01
Published in Childs Nervous System. New York: Springer, v. 25, n. 1, p. 39-45, 2009.
ISSN 0256-7040 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 39-45
Access rights Closed access
Type Article
Web of Science ID WOS:000261374800007

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