Increases of kinin B-1 and B-2 receptors binding sites after brain infusion of amyloid-beta 1-40 peptide in rats

Increases of kinin B-1 and B-2 receptors binding sites after brain infusion of amyloid-beta 1-40 peptide in rats

Author Viel, Tania Araujo Google Scholar
Caetano, Ariadiny Lima Google Scholar
Nasello, Antonia Gladys Google Scholar
Lancelotti, Carmen Lucia Google Scholar
Nunes, Viviane Abreu Autor UNIFESP Google Scholar
Araujo, Mariana Silva Autor UNIFESP Google Scholar
Buck, Hudson Sousa Google Scholar
Institution Fac Ciencias Med Santa Casa
Universidade Federal de São Paulo (UNIFESP)
Abstract Although numerous inflammation pathways have been implicated in Alzheimer's disease, the involvement of the kallikrein-kinin system is still under investigation. We anatomically localized and quantified the density of kinin B-1 and B-2 receptors binding sites in the rat brain after the infusion of amyloid-beta (A beta) peptide in the right lateral brain ventricle for 5 weeks. the conditioned avoidance test showed a significant reduction of memory consolidation in rats infused with A beta (68.6 +/- 20.9%, P < 0.05) when compared to control group (90.8 +/- 4.1%; infused with vehicle). Autoradiographic studies performed in brain samples of both groups using [I-125]HPP-[des-Arg(10)]-Hoe-140 (150 pM, 90 min, 25 degrees C) showed a significant increase in density of B-1 receptor binding sites in the ventral hippocampal commissure (1.23 +/- 0.07 fmol/mg), fimbria (1.31 +/- 0.05 fmol/mg), CA1 and CA3 hippocampal areas (1.05 +/- 0.03 and 1.24 +/- 0.02 fmol/mg, respectively), habenular nuclei (1.30 +/- 0.04 fmol/mg), optical tract (1.30 +/- 0.05 fmol/mg) and internal capsule (1.26 +/- 0.05 fmol/mg) in A beta group. for B-2 receptors ([I-125]HPP-Hoe-140, 200 pM, 90 min, 25 degrees C), a significant increase in density of binding sites was observed in optical tract (2.04 +/- 0.08 fmol/mg), basal nucleus of Meynert (1.84 +/- 0.18 fmol/mg), lateral septal nucleus - dorsal and intermediary portions (1.66 +/- 0.29 fmol/mg), internal capsule (1.74 +/- 0.19 fmol/mg) and habenular nuclei (1.69 +/- 0.11 fmol/mg). in control group, none of these nuclei showed [I-125]HPP-Hoe-140 labeling. This significant increase in densities of kinin B-1 and B-2 receptors in animals submitted to A beta infusion was observed mainly in brain regions related to cognitive behavior, suggesting the involvement of the kallikrein-kinin system in Alzheimer's disease in vivo. (c) 2007 Elsevier Inc. All rights reserved.
Keywords Bradykinin
Neuroinflammation
Cognitive function
Neurodegeneration
Amyloid-beta infusion
B-1 and B-2 receptors
Language English
Sponsor Fundo de Amparo a Pesquisa da Faculdade de Ciencias Medicas da Santa Casa de São Paulo (FAP-FCM)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2008-12-01
Published in Neurobiology of Aging. New York: Elsevier B.V., v. 29, n. 12, p. 1805-1814, 2008.
ISSN 0197-4580 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1805-1814
Origin http://dx.doi.org/10.1016/j.neurobiolaging.2007.04.019
Access rights Closed access
Type Article
Web of Science ID WOS:000260718700005
URI http://repositorio.unifesp.br/handle/11600/31081

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