Bothrops jararaca fibrinogen and its resistance to hydrolysis evoked by snake venoms

Bothrops jararaca fibrinogen and its resistance to hydrolysis evoked by snake venoms

Autor Vieira, Carolina O. Google Scholar
Tanaka, Aparecida S. Autor UNIFESP Google Scholar
Sano-Martins, Ida S. Google Scholar
Morais, Karen B. Google Scholar
Santoro, Marcelo L. Google Scholar
Tanaka-Azevedo, Anita M. Google Scholar
Instituição Inst Butantan
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo Fibrinogen is an essential protein involved in several steps of hemostasis, being associated with the final steps of the blood coagulation mechanism. Herein, we describe the purification and characterization of a reptile fibrinogen, obtained from Bothrops jararaca plasma. Native B. jararaca fibrinogen showed a molecular mass of 372 kDa, and the reduced and alkylated fibrinogen molecule showed three chains of 71, 60 and 55 kDa, which are similar to the molecular masses of human and bovine Act, BP and gamma fibrinogen chains. Remarkably, B. jararaca fibrinogen was clotted by bovine thrombin, but B. jararaca, Crotalus durissus terrificus and Lachesis muta rhombeata venoms could not induce its clotting or hydrolysis. Thus, despite the similarities between B. jararaca and mammalian fibrinogens, the former shows distinctive features, which protect B. jararaca snakes from accidental envenomation. (c) 2008 Elsevier Inc. All rights reserved.
Palavra-chave Bothrops jararaca
Blood coagulation
Fibrinogen
Reptiles
Snake plasma
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento FAPESP: 04/02224-4
Data de publicação 2008-12-01
Publicado em Comparative Biochemistry and Physiology B-biochemistry & Molecular Biology. New York: Elsevier B.V., v. 151, n. 4, p. 428-432, 2008.
ISSN 1096-4959 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 428-432
Fonte http://dx.doi.org/10.1016/j.cbpb.2008.08.012
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000261672100009
Endereço permanente http://repositorio.unifesp.br/handle/11600/31069

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