Expression of functional recombinant human factor IX in milk of mice

Expression of functional recombinant human factor IX in milk of mice

Autor Lisauskas, Sharon F. C. Google Scholar
Cunha, Nicolau B. Google Scholar
Vianna, Giovanni R. Google Scholar
Mendes, Erica A. Google Scholar
Ramos, Gustavo L. Google Scholar
Maranhao, Andreia Q. Google Scholar
Brigido, Marcelo M. Google Scholar
Almeida, Jussara O. S. C. Google Scholar
Baptista, Heloisa A. Autor UNIFESP Google Scholar
Motta, Fabiana L. T. Autor UNIFESP Google Scholar
Pesquero, Joao B. Autor UNIFESP Google Scholar
Aragao, Francisco J. L. Google Scholar
Rech, Elibio L. Google Scholar
Instituição Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade de Brasília (UnB)
SGAIN
Universidade Federal de São Paulo (UNIFESP)
Resumo Human factor IX is synthesized in the liver and secreted in the blood, where it participates in a group of reactions involving coagulation factors and proteins that permit sanguinary coagulation. in this work two lines of transgenic mice were developed to express the FIX gene in the mammalian glands under control of milk beta-casein promoter. the founding females secreted the FIX in their milk (3% total soluble protein). the stable integration of transgene was confirmed by southern blot analysis. the presence of the FIX recombinant protein in the milk of transgenic females was confirmed by western blot and the clotting activity was revealed in blood-clotting assays. the coagulation activity in human blood treated with recombinant FIX increased while the time of coagulation decreased. Our results confirm the production of a large amount of recombinant biologically active FIX in the mammary gland of transgenic mice.
Palavra-chave Expression
Recombinant human factor IX
Transgenic mice
Idioma Inglês
Financiador Empresa Brasileira de Pesquisa Agropecuaria (Embrapa)
Hospital de Apoio de Brasilia
Universidade Federal de São Paulo
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
International Hemophilia Training Center-World Federation of Hemophilia
Data de publicação 2008-12-01
Publicado em Biotechnology Letters. Dordrecht: Springer, v. 30, n. 12, p. 2063-2069, 2008.
ISSN 0141-5492 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 2063-2069
Fonte http://dx.doi.org/10.1007/s10529-008-9818-y
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000260306800004
Endereço permanente http://repositorio.unifesp.br/handle/11600/31064

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