Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop

Molecular and structural characterization of HIV-1 subtype B Brazilian isolates with GWGR tetramer at the tip of the V3-loop

Author Leal, Elcio de Souza Autor UNIFESP Google Scholar
Silva, Wilson Pereira Autor UNIFESP Google Scholar
Sucupira, Maria Cecília Araripe Autor UNIFESP Google Scholar
Janini, Luiz Mário Ramos Autor UNIFESP Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract One of most intriguing features of the HIV-1 subtype B epidemic in Brazil is the high frequency of isolates exhibiting tryptophan (W) in the tetramer (GWGR) at the tip of the V3 loop. We observed that the frequencies of glutamic and aspartic acids at site 25 of the V3 loop are quite distinct in GWGR isolates compared with viruses with other tetramers. the basic amino acids at sites 11 and 25 of V3 are strongly linked with CCR5-to-CXCR4 coreceptor shift. We therefore predicted phenotype usage and found that GWGR isolates are exclusively CCR5-using. Further evidence of this came from intrahost sequences, where basic amino acid substitutions at sites 11 and 25 emerged only in isolates presenting a tryptophan-to-glycine replacement at the tetramer of the V3. in addition, modeled 3D-structures of the V3 loop of GWGR and GGGR in intrahost viruses differ essentially in the binding region of the coreceptor. (C) 2008 Elsevier Inc. All rights reserved.
Keywords HIV-1
V3 loop
GWGR
Coreceptor
CCR5
CXCR4
3D-structure
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/10372-3
FAPESP: 07/52841-8
Date 2008-11-25
Published in Virology. San Diego: Academic Press Inc Elsevier Science, v. 381, n. 2, p. 222-229, 2008.
ISSN 0042-6822 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 222-229
Origin http://dx.doi.org/10.1016/j.virol.2008.08.029
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000260841500009
URI http://repositorio.unifesp.br/handle/11600/31045

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