Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Changes in Intracellular Ca2+ Levels Induced by Cytokines and P2 Agonists Differentially Modulate Proliferation or Commitment with Macrophage Differentiation in Murine Hematopoietic Cells

Autor Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Leon, Carlos M. M. P. Autor UNIFESP Google Scholar
Borojevic, Radovan Google Scholar
Oshiro, Maria Etsuko Miyamoto Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Resumo The role of intracellular Ca2+ (Ca-i(2+)) on hematopoiesis was investigated in long term bone marrow cultures using cytokines and agonists of P2 receptors. Cytokines interleukin 3 and granulocyte/macrophage colony stimulator factor promoted a modest increase in Ca-i(2+) concentration ([Ca2+](i)) with activation of phospholipase C gamma, MEK1/2, and Ca2+/calmodulin kinase II. Involvement of protein kinase C was restricted to stimulation with interleukin 3. in addition, these cytokines promoted proliferation (20 times) and an increase in the Gr-1(-)Mac-1(+) population with participation of gap junctions (GJ). Nevertheless ATP, ADP, and UTP promoted a large increase in [Ca2+](i), moderate proliferation (6 times), a reduction in the primitive Gr-1(-)Mac-1(-)c-Kit(+) population, and differentiation into macrophages without participation of GJ. It is likely that Ca-i(2+) participates as a regulator of hematopoietic signaling: moderate increases in [Ca2+](i) would be related to cytokine-dependent proliferation with participation of GJ, whereas high increases in [Ca2+](i) would be related to macrophage differentiation without maintenance of the primitive population.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Data 2008-11-14
Publicado em Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 283, n. 46, p. 31909-31919, 2008.
ISSN 0021-9258 (Sherpa/Romeo, fator de impacto)
Editor Amer Soc Biochemistry Molecular Biology Inc
Extensão 31909-31919
Fonte http://dx.doi.org/10.1074/jbc.M801990200
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000260760800073
URI http://repositorio.unifesp.br/handle/11600/31036

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