Effects of the venom and the dermonecrotic toxin LiRecDT1 of Loxosceles intermedia in the rat liver

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dc.contributor.author Christoff, Adriana de Oliveira
dc.contributor.author Oliveira, Anabel de
dc.contributor.author Chaim, Olga Meiri [UNIFESP]
dc.contributor.author Lugarini, Daiana
dc.contributor.author Pereira, Amanda Leite Bastos
dc.contributor.author Paludo, Katia Sabrina [UNIFESP]
dc.contributor.author Telles, Jose Ederaldo Queiroz
dc.contributor.author Bracht, Adelar
dc.contributor.author Veiga, Silvio Sanches
dc.contributor.author Acco, Alexandra
dc.date.accessioned 2016-01-24T13:51:52Z
dc.date.available 2016-01-24T13:51:52Z
dc.date.issued 2008-11-01
dc.identifier http://dx.doi.org/10.1016/j.toxicon.2008.08.001
dc.identifier.citation Toxicon. Oxford: Pergamon-Elsevier B.V., v. 52, n. 6, p. 695-704, 2008.
dc.identifier.issn 0041-0101
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31015
dc.description.abstract Brown spider bites cause dermonecrotic lesions and systemic manifestations known as loxoscelism. the Loxosceles intermedia venom contains many active proteins, as phospholipase D. There are reports of increased levels of hepatic transaminases in humans with loxoscelism, but detailed studies about the action of the Loxosceles intermedia venom on the liver functions are lacking. the aim of this study was to investigate the effects of the venom and the dermonecrotic recombinant toxin 1 (LiRecDT1) in the liver of Wistar rats injected subcutaneously with venom (80 mu g) or toxin (80 mu g). After 6 and 12 in the liver immunofluorescence was positive for venom and toxin. Hepatocytes from the venom group were tumefacted and apoptotic. There was leucocyte infiltration in the portal region combined with a high degree of steatosis in 12 h. in the toxin group the histological alterations were less severe. Plasma levels of alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl-transferase were significantly elevated only in the venom group in 6 h. Hepatic metabolism was modified: the venom, but not LiRecDT1, reduced gluconeogenesis and ureagenesis from alanine and glycogen accumulation. These results show that the venom is hepatortoxic and that the dermonecrotic toxin is only partly responsible. (c) 2008 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundacao Araucaria-PR/PPSUS
dc.description.sponsorship Secretaria de Estado de Ciencia
dc.description.sponsorship Tecnologia e Ensino Superior (SETI) do Parana
dc.format.extent 695-704
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Toxicon
dc.rights Acesso restrito
dc.subject Brown spider en
dc.subject Loxosceles intermedia en
dc.subject Loxoscelism en
dc.subject Liver en
dc.subject Metabolism en
dc.title Effects of the venom and the dermonecrotic toxin LiRecDT1 of Loxosceles intermedia in the rat liver en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Univ Fed Parana
dc.contributor.institution Universidade Estadual de Maringá (UEM)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Parana, Dept Pharmacol, BR-81531990 Curitiba, Parana, Brazil
dc.description.affiliation Univ Fed Parana, Dept Cell Biol, BR-81531990 Curitiba, Parana, Brazil
dc.description.affiliation Univ Fed Parana, Dept Med Pathol, BR-81531990 Curitiba, Parana, Brazil
dc.description.affiliation Univ Estadual Maringa, Dept Biochem, Maringa, Parana, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Clin Med, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biochem, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Clin Med, São Paulo, Brazil
dc.identifier.doi 10.1016/j.toxicon.2008.08.001
dc.description.source Web of Science
dc.identifier.wos WOS:000261013300005



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