Effects of the venom and the dermonecrotic toxin LiRecDT1 of Loxosceles intermedia in the rat liver

Effects of the venom and the dermonecrotic toxin LiRecDT1 of Loxosceles intermedia in the rat liver

Author Christoff, Adriana de Oliveira Google Scholar
Oliveira, Anabel de Google Scholar
Chaim, Olga Meiri Autor UNIFESP Google Scholar
Lugarini, Daiana Google Scholar
Pereira, Amanda Leite Bastos Google Scholar
Paludo, Katia Sabrina Autor UNIFESP Google Scholar
Telles, Jose Ederaldo Queiroz Google Scholar
Bracht, Adelar Google Scholar
Veiga, Silvio Sanches Google Scholar
Acco, Alexandra Google Scholar
Institution Univ Fed Parana
Universidade Estadual de Maringá (UEM)
Universidade Federal de São Paulo (UNIFESP)
Abstract Brown spider bites cause dermonecrotic lesions and systemic manifestations known as loxoscelism. the Loxosceles intermedia venom contains many active proteins, as phospholipase D. There are reports of increased levels of hepatic transaminases in humans with loxoscelism, but detailed studies about the action of the Loxosceles intermedia venom on the liver functions are lacking. the aim of this study was to investigate the effects of the venom and the dermonecrotic recombinant toxin 1 (LiRecDT1) in the liver of Wistar rats injected subcutaneously with venom (80 mu g) or toxin (80 mu g). After 6 and 12 in the liver immunofluorescence was positive for venom and toxin. Hepatocytes from the venom group were tumefacted and apoptotic. There was leucocyte infiltration in the portal region combined with a high degree of steatosis in 12 h. in the toxin group the histological alterations were less severe. Plasma levels of alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl-transferase were significantly elevated only in the venom group in 6 h. Hepatic metabolism was modified: the venom, but not LiRecDT1, reduced gluconeogenesis and ureagenesis from alanine and glycogen accumulation. These results show that the venom is hepatortoxic and that the dermonecrotic toxin is only partly responsible. (c) 2008 Elsevier B.V. All rights reserved.
Keywords Brown spider
Loxosceles intermedia
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundacao Araucaria-PR/PPSUS
Secretaria de Estado de Ciencia
Tecnologia e Ensino Superior (SETI) do Parana
Date 2008-11-01
Published in Toxicon. Oxford: Pergamon-Elsevier B.V., v. 52, n. 6, p. 695-704, 2008.
ISSN 0041-0101 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 695-704
Origin http://dx.doi.org/10.1016/j.toxicon.2008.08.001
Access rights Closed access
Type Article
Web of Science ID WOS:000261013300005
URI http://repositorio.unifesp.br/handle/11600/31015

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