Can the rDNA methylation pattern be used as a marker for Alzheimer's disease?

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dc.contributor.author Speranca, Marcia Aparecida
dc.contributor.author Batista, Lisandra Mesquita
dc.contributor.author Lourenco, Ricardo da Silva
dc.contributor.author Tavares, Wagner Malago
dc.contributor.author Bertolucci, Paulo Henrique Ferreira [UNIFESP]
dc.contributor.author Santos Rigolin, Valdeci de Oliveira
dc.contributor.author Payão, Spencer Luiz Marques [UNIFESP]
dc.contributor.author Smith, Marilia de Arruda Cardoso [UNIFESP]
dc.date.accessioned 2016-01-24T13:51:51Z
dc.date.available 2016-01-24T13:51:51Z
dc.date.issued 2008-11-01
dc.identifier http://dx.doi.org/10.1016/j.jalz.2008.03.010
dc.identifier.citation Alzheimers & Dementia. New York: Elsevier B.V., v. 4, n. 6, p. 438-442, 2008.
dc.identifier.issn 1552-5260
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/31009
dc.description.abstract Background: Differential methylation activity of the human rDNA in Alzheimer's disease (AD) patients has been demonstrated by classic cytogenetic tools. indicating a decrease in rRNA gene expression. Methylation of CpGs is an important epigenetic mechanism involved in gene expression repression of tandem repeating genes during ageing. Thus, rDNA specific methylation pattern could be involved in AD and be used as a marker of the disease or of its progression.Methods: the methylation pattern of three rDNA regions, including the promoter, 18S, and 28S, was investigated with the use of restriction endonucleases sensitive to methylation and Southern blotting, from DNA extracted from total peripheral blood cells of 28 AD patients and 28 elderly and Young controls.Results: We did not find a significant divergence in the methylation pattern of the Studied regions and in the relative amount of rDNA methylated copies among the individuals' groups.Conclusions: No differential methylation pattern of rDNA genes was observed in total peripheral blood cells in aged and AD subjects by the methodology used. (C) 2008 the Alzheimer's Association, All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 438-442
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Alzheimers & Dementia
dc.rights Acesso restrito
dc.subject Analysis of the human rDNA methylation pattern in ageing and Alzheimer's disease en
dc.subject Human rDNA en
dc.subject Alzheimer's disease en
dc.subject Ageing en
dc.subject rRNA expression en
dc.title Can the rDNA methylation pattern be used as a marker for Alzheimer's disease? en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Marilia Med Sch
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Marilia Med Sch, Disciplina Biol Mol, Marilia, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol, São Paulo, Brazil
dc.description.affiliation Marilia Med Sch, Disciplina Geriatria, Marilia, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Genet, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Neurol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Disciplina Genet, São Paulo, Brazil
dc.identifier.doi 10.1016/j.jalz.2008.03.010
dc.description.source Web of Science
dc.identifier.wos WOS:000261056800010



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