Can the rDNA methylation pattern be used as a marker for Alzheimer's disease?

Can the rDNA methylation pattern be used as a marker for Alzheimer's disease?

Author Speranca, Marcia Aparecida Google Scholar
Batista, Lisandra Mesquita Google Scholar
Lourenco, Ricardo da Silva Google Scholar
Tavares, Wagner Malago Google Scholar
Bertolucci, Paulo Henrique Ferreira Autor UNIFESP Google Scholar
Santos Rigolin, Valdeci de Oliveira Google Scholar
Payão, Spencer Luiz Marques Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Institution Marilia Med Sch
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Differential methylation activity of the human rDNA in Alzheimer's disease (AD) patients has been demonstrated by classic cytogenetic tools. indicating a decrease in rRNA gene expression. Methylation of CpGs is an important epigenetic mechanism involved in gene expression repression of tandem repeating genes during ageing. Thus, rDNA specific methylation pattern could be involved in AD and be used as a marker of the disease or of its progression.Methods: the methylation pattern of three rDNA regions, including the promoter, 18S, and 28S, was investigated with the use of restriction endonucleases sensitive to methylation and Southern blotting, from DNA extracted from total peripheral blood cells of 28 AD patients and 28 elderly and Young controls.Results: We did not find a significant divergence in the methylation pattern of the Studied regions and in the relative amount of rDNA methylated copies among the individuals' groups.Conclusions: No differential methylation pattern of rDNA genes was observed in total peripheral blood cells in aged and AD subjects by the methodology used. (C) 2008 the Alzheimer's Association, All rights reserved.
Keywords Analysis of the human rDNA methylation pattern in ageing and Alzheimer's disease
Human rDNA
Alzheimer's disease
Ageing
rRNA expression
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2008-11-01
Published in Alzheimers & Dementia. New York: Elsevier B.V., v. 4, n. 6, p. 438-442, 2008.
ISSN 1552-5260 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 438-442
Origin http://dx.doi.org/10.1016/j.jalz.2008.03.010
Access rights Closed access
Type Article
Web of Science ID WOS:000261056800010
URI http://repositorio.unifesp.br/handle/11600/31009

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