Pre-treatment of cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo development

Pre-treatment of cattle semen or oocytes with purified milk osteopontin affects in vitro fertilization and embryo development

Autor Goncalves, R. F. Google Scholar
Chapman, D. A. Google Scholar
Bertolla, R. P. Autor UNIFESP Google Scholar
Eder, I. Google Scholar
Killian, G. J. Google Scholar
Instituição Universidade de São Paulo (USP)
Penn State Univ
Universidade Federal de São Paulo (UNIFESP)
Resumo This study was designed to investigate the effects of pre-incubating cattle spermatozoa or matured oocytes with purified osteopontin (OPN) from cattle milk on fertilization in cattle and embryonic development in vitro. There were two different experiments, semen from six mature Holstein bulls (Bos Taurus) was frozen with different concentrations of OPN (0, 1, 10, 100 mu g/mL). Matured cattle oocytes were also pre-treated with OPN (0, 10, 100 mu g/mL). in both experiments, pre-treated oocytes or frozen semen, was processed for in vitro fertilization and embryo development. Significantly more oocytes were fertilized when using frozen semen with 10 mu g/mL OPN (bull 2 = 85 +/- 4% and bull 5 = 78 +/- 4%) than without OPN (bull 2 = 75 +/- 4% and bull 5 = 69 +/- 4%). Those bulls also had increase in cleavage and embryo development (bull 2 = 85 +/- 3%, 41 +/- 1.9%; bull 5 = 76 +/- 2%, 37 +/- 1.8%) compared with control (bull 2 = 75 +/- 3%, 30 +/- 2%; bull 5 = 68 +/- 2%, 29 +/- 2%). Incubating matured oocytes in 10 mu g/mL OPN (87 +/- 3%) and 100 mu g/mL OPN (88 +/- 3%) significantly increased fertilization than control (73 +/- 3%). OPN also improve cleavage, and embryo development in treatments with 10 mu g/mL OPN (82.7 +/- 1.3%; 31.7 +/- 1.4%) and 100 mu g/mL OPN (85.8 +/- 1.3%; 33.8 +/- 1.5%) when compared with control (74.1 +/- 1.3%; 24.2 +/- 1.2%). These data suggest that both, spermatozoa from some bulls and oocytes may associate with OPN, suggesting a facilitory role on in vitro fertilization and embryo development. (C) 2007 Elsevier B.V. All rights reserved.
Assunto Osteopontin (OPN)
Secreted phosphoprotein 1 (SPP1)
In vitro
Cattle
Fertilization
Embryo development
Idioma Inglês
Financiador USDA
Número do financiamento USDA: 2001-34281-11219
USDA: 2002-34437-11771
USDA: 2003-34437-13460
Data 2008-11-01
Publicado em Animal Reproduction Science. Amsterdam: Elsevier B.V., v. 108, n. 3-4, p. 375-383, 2008.
ISSN 0378-4320 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 375-383
Fonte http://dx.doi.org/10.1016/j.anireprosci.2007.09.006
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000259883700011
URI http://repositorio.unifesp.br/handle/11600/31001

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