Correlation Between Autofluorescence Intensity and Tumor Area in Mice Bearing Renal Cell Carcinoma

Correlation Between Autofluorescence Intensity and Tumor Area in Mice Bearing Renal Cell Carcinoma

Author Bellini, Maria Helena Autor UNIFESP Google Scholar
Coutinho, Enia Lucia Autor UNIFESP Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Silva, Flávia Rodrigues de Oliveira Autor UNIFESP Google Scholar
Vieira, Nilson Dias Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
IPEN CNEN SP
Universidade de São Paulo (USP)
Abstract Protoporphyrin IX (PpIX) is a porphyrin derivative that is accumulated in cancerous tissue in consequence of the tumor-specific metabolic alterations. the aim of this study was to evaluate the accumulation of PpIX in mice bearing renal cell carcinoma by spectroscopy analysis. A total of 24 male Balb/c mice, 6 weeks old, were divided into six groups: Normal (without inoculation of tumor cells) and 4, 8, 13, 16, and 20 days after inoculation of tumor cells. the orthotopic tumor model of renal cancer was used. Murine renal cell carcinoma (Renca cells) were inoculated into the subcapsular space of the kidney. Normal and tumor-bearing kidneys in different progression stages were removed and analyzed by ex-vivo spectroscopy and by microscopy, for tumor histometric analysis. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and tumor-bearing kidneys in autofluorescence shape occurred in the 600-700 nm spectral region. A good correlation was found between emission band intensity at 635 nm and the tumor area.
Keywords Renal carcinoma
Porphyrin
Fluorescence
Tumor growth
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 03/11779-9
Date 2008-11-01
Published in Journal of Fluorescence. New York: Springer/plenum Publishers, v. 18, n. 6, p. 1163-1168, 2008.
ISSN 1053-0509 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 1163-1168
Origin http://dx.doi.org/10.1007/s10895-008-0368-4
Access rights Closed access
Type Article
Web of Science ID WOS:000262834700018
URI http://repositorio.unifesp.br/handle/11600/30996

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