Internalization and degradation of heparin is not required for stimulus of heparan sulfate proteoglycan synthesis

Internalization and degradation of heparin is not required for stimulus of heparan sulfate proteoglycan synthesis

Autor Trindade, Edvaldo S. Google Scholar
Boucas, Rodrigo I. Google Scholar
Rocha, Hugo A. O. Google Scholar
Dominato, Juliana Augusta Albieri Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar J. Google Scholar
Franco, Celia Regina C. Google Scholar
Oliver, Constance Google Scholar
Jamur, Maria C. Google Scholar
Dietrich, Carl Peter Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Univ Fed Rio Grande do Norte
Universidade de São Paulo (USP)
Resumo In vitro, heparin and antithrombotic drugs specifically stimulate the synthesis of an antithrombotic heparan sulfate proteoglycan (HSPG) produced by endothelial cells. the putative heparin binding site(s) that may be related to this phenomenon were investigated. in the preceding article, using various heparin probes, it was shown that the heparin does not bind to the endothelial cell surface, but only to the extracellular matrix. the present study demonstrated that, when the cells were exposed to heparin at 37 C, the heparin was internalized and with time was localized in lysosomes. However, endocytosis of heparin was not required for the stimulation of HSPG synthesis. the requirement for heparin degradation in the stimulus of HSPG synthesis was also investigated. When the cells were incubated with chloroquine, a lysosomotropic amine that raises the lysosomal pH thus inhabiting enzymatic degradation of internalized compounds, stimulation of HSPG synthesis was still observed. These combined results indicate that neither internalization nor degradation of heparin is required for stimulation of HSPG synthesis, and suggests that its binding to the extracellular matrix could be responsible for this effect.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Financiadora de Estudos e Projetos (FINEP), Brazil
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2008-11-01
Publicado em Journal of Cellular Physiology. Hoboken: Wiley-liss, v. 217, n. 2, p. 360-366, 2008.
ISSN 0021-9541 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 360-366
Fonte http://dx.doi.org/10.1002/jcp.21510
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000259721800009
Endereço permanente http://repositorio.unifesp.br/handle/11600/30988

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