Sperm nuclear apoptotic DNA fragmentation in men with testicular cancer

Sperm nuclear apoptotic DNA fragmentation in men with testicular cancer

Autor Ribeiro, Taisa Michelucci Autor UNIFESP Google Scholar
Bertolla, Ricardo Pimenta Autor UNIFESP Google Scholar
Spaine, Deborah Montagnini Autor UNIFESP Google Scholar
Fraietta, Renato Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Objective: To verify whether sperm from patients with a semi noma and patients with a non-semi noma present with an increased rate of apoptotic DNA fragmentation, when compared with men without testicular cancer and who had fathered a child in the 2 years preceding the study.Design: Controlled prospective study.Setting: Patients referred to a sperm bank in an academic research environment.Patient(s): Men with a diagnosed seminoma, men with a diagnosed non-seminoma, both after orchiectomy and before adjuvant therapy, and men with proven paternity in the 2 previous years.Main Outcome Measure(s): Rate of nuclear apoptotic sperm DNA fragmentation as assessed by TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) assay, classified as positive (with DNA fragmentation) or negative (without DNA fragmentation).Result(s): of the 48 men with testicular cancer included in the study, 29 (60.4%) presented a non-seminoma and 19 (39.6%) a seminoma. Patients with non-seminoma presented with lower progressive sperm motility than the control group (57.4% and 66.3%, respectively), but both were still within normal ranges. Sperm concentration was lower in seminoma (31.2 x 10(6)/mL) and in non-seminoma (20.6 x 10(6)/mL) when compared with the control group (78.1 x 10(6)/mL), but values did not differ between the two testicular cancer groups. Sperm morphology was lower in patients with non-seminoma than in the control group (10% and 13.1%, respectively). Results for sperm nuclear apoptotic DNA fragmentation (mean; standard deviation) were 12.6%; 4.5% for the control group, 12.2%; 5.5% for the non-seminoma group, and 12.5%; 6.4% for the seminoma group. No differences were found between the three groups.Conclusion(s): Our results demonstrate that the presence of a seminoma or a non-seminoma is not associated with an increase in sperm apoptotic DNA fragmentation. (Fertil Steril((R)) 2008;90:1782-6. (c) 2008 by American Society for Reproductive Medicine.)
Palavra-chave Testicular cancer
infertility
sperm
DNA fragmentation
TUNEL assay
Idioma Inglês
Data de publicação 2008-11-01
Publicado em Fertility and Sterility. New York: Elsevier B.V., v. 90, n. 5, p. 1782-1786, 2008.
ISSN 0015-0282 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 1782-1786
Fonte http://dx.doi.org/10.1016/j.fertnstert.2007.08.012
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000260752000032
Endereço permanente http://repositorio.unifesp.br/handle/11600/30975

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