Urinary glycosaminoglycans as biomarker for urothelial injury: Is it possible to discriminate damage from recovery?

Urinary glycosaminoglycans as biomarker for urothelial injury: Is it possible to discriminate damage from recovery?

Autor Soler, Roberto Google Scholar
Bruschini, Homero Google Scholar
Martins, Joao R. Google Scholar
Dreyfuss, Juliana L. Google Scholar
Camara, Niels O. Google Scholar
Alves, Maria T. Google Scholar
Leite, Katia R. Google Scholar
Truzzi, Jose C. Google Scholar
Nader, Helena B. Google Scholar
Srougi, Miguel Google Scholar
Ortiz, Vaidemar Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo OBJECTIVES the glycosaminoglycan (GAG) layer is referred to as a bladder protective factor. We reproduced an experimental model of urothelial damage to assess GAG metabolism in the process of injury and recovery of the urothelium.METHODS Wistar female rats were bladder catheterized and instilled with either protamine sulfate (PS groups) or sterile saline (control groups). At different days after the procedure, 24-hour urine samples were obtained. the urinary levels of hyaluronic acid (HA) and sulfated glycosaminoglycan were determined in all groups and in nonmanipulated rats (day 0). Additionally, sulfated-GAG synthesis was assessed by the incorporation of [S-35]-inorganic sulfate. the bladders were analyzed by histochemical staining for HA and immunofluorescence for heparin sulfate and syndecan-4.RESULTS Urinary HA and sulfated-GAG were elevated after PS injection (P <0.05). A greater concentration of [S-35] -labeled GAG in the PS group animals on the fifth day and, especially, on the seventh day represented increased GAG synthesis at these periods (P <0.05). Bladder sections from the PS group animals on day 1 showed a greater amount of HA in the urothelium. PS instillation damaged the urothelium layer of heparin sulfate and syndecan-4 seen in the control animals. On day 5, patchy areas of a restored layer were seen, and, on day 7, this layer had completely regenerated.CONCLUSIONS Urinary GAG cannot differentiate urothelial damage from recovery. Elevated levels of urinary GAG can result from either desquamation of the surface cell GAG layer or increased GAG synthesis to regenerate the damaged urothelium.
Idioma Inglês
Data de publicação 2008-10-01
Publicado em Urology. New York: Elsevier B.V., v. 72, n. 4, p. 937-942, 2008.
ISSN 0090-4295 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 937-942
Fonte http://dx.doi.org/10.1016/j.urology.2008.01.028
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000259845600053
Endereço permanente http://repositorio.unifesp.br/handle/11600/30953

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