Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

Author Mufalo, Bruno C. Google Scholar
Gentil, Fernanda Google Scholar
Bargieri, Daniel Y. Autor UNIFESP Google Scholar
Costa, Fabio Trindade Maranhão Autor UNIFESP Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Soares, Irene S. Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Abstract The Apical Membrane Antigen-1 (AMA-1) of Plasmodium sp. has been suggested as a vaccine candidate against malaria. This protein seems to be involved in merozoite invasion and its extra-cellular portion contains three distinct domains: DI, DII, and DIII. Previously, we described that Plasmodium vivax AMA-1 (PvAMA-1) ectodomain is highly immunogenic in natural human infections. Here, we expressed each domain, separately or in combination (DI-II or DII-III), as bacterial recombinant proteins to map immunodominant epitopes within the PvAMA-1 ectodomain. IgG recognition was assessed by ELISA using sera of P. vivax-infected individuals collected from endemic regions of Brazil or antibodies raised in immunized mice. the frequencies of responders to recombinant proteins containing the DII were higher than the others and similar to the ones observed against the PvAMA-1 ectodomain. Moreover, ELISA inhibition assays using the PvAMA-1 ectodomain as substrate revealed the presence of many common epitopes within DI-II that are recognized by human immune antibodies. Finally, immunization of mice with the PvAMA-1 ectodomain induced high levels of antibodies predominantly to DI-II. Together, our results indicate that DII is particularly immunogenic during natural human infections, thus indicating that this region could be used as part of an experimental sub-unit vaccine to prevent vivax malaria. (C) 2008 Elsevier Masson SAS. All rights reserved.
Keywords Malaria
Plasmodium vivax
Apical Membrane Antigen-1
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2004/00768-7
FAPERJ: E-26/110.305/2007
CNPq: 420067/2005-1
Date 2008-10-01
Published in Microbes and Infection. Amsterdam: Elsevier B.V., v. 10, n. 12-13, p. 1266-1273, 2008.
ISSN 1286-4579 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1266-1273
Origin http://dx.doi.org/10.1016/j.micinf.2008.07.023
Access rights Closed access
Type Article
Web of Science ID WOS:000261368400006
URI http://repositorio.unifesp.br/handle/11600/30942

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