Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

Plasmodium vivax apical membrane antigen-1: comparative recognition of different domains by antibodies induced during natural human infection

Autor Mufalo, Bruno C. Google Scholar
Gentil, Fernanda Google Scholar
Bargieri, Daniel Y. Autor UNIFESP Google Scholar
Costa, Fabio Trindade Maranhão Autor UNIFESP Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Soares, Irene S. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Resumo The Apical Membrane Antigen-1 (AMA-1) of Plasmodium sp. has been suggested as a vaccine candidate against malaria. This protein seems to be involved in merozoite invasion and its extra-cellular portion contains three distinct domains: DI, DII, and DIII. Previously, we described that Plasmodium vivax AMA-1 (PvAMA-1) ectodomain is highly immunogenic in natural human infections. Here, we expressed each domain, separately or in combination (DI-II or DII-III), as bacterial recombinant proteins to map immunodominant epitopes within the PvAMA-1 ectodomain. IgG recognition was assessed by ELISA using sera of P. vivax-infected individuals collected from endemic regions of Brazil or antibodies raised in immunized mice. the frequencies of responders to recombinant proteins containing the DII were higher than the others and similar to the ones observed against the PvAMA-1 ectodomain. Moreover, ELISA inhibition assays using the PvAMA-1 ectodomain as substrate revealed the presence of many common epitopes within DI-II that are recognized by human immune antibodies. Finally, immunization of mice with the PvAMA-1 ectodomain induced high levels of antibodies predominantly to DI-II. Together, our results indicate that DII is particularly immunogenic during natural human infections, thus indicating that this region could be used as part of an experimental sub-unit vaccine to prevent vivax malaria. (C) 2008 Elsevier Masson SAS. All rights reserved.
Palavra-chave Malaria
Plasmodium vivax
Apical Membrane Antigen-1
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Número do financiamento FAPESP: 2004/00768-7
FAPERJ: E-26/110.305/2007
CNPq: 420067/2005-1
Data de publicação 2008-10-01
Publicado em Microbes and Infection. Amsterdam: Elsevier B.V., v. 10, n. 12-13, p. 1266-1273, 2008.
ISSN 1286-4579 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 1266-1273
Fonte http://dx.doi.org/10.1016/j.micinf.2008.07.023
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000261368400006
Endereço permanente http://repositorio.unifesp.br/handle/11600/30942

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