Diversity of virulence profiles of Shiga toxin-producing Escherichia coli serotypes in food-producing animals in Brazil

Diversity of virulence profiles of Shiga toxin-producing Escherichia coli serotypes in food-producing animals in Brazil

Autor Oliveira, Murilo Gomes Google Scholar
Feitosa Brito, Jose Renaldi Google Scholar
Tardelli Gomes, Tania Aparecida Autor UNIFESP Google Scholar
Cabilio Guth, Beatriz Ernestina Autor UNIFESP Google Scholar
Midolli Vieira, Monica Aparecida Autor UNIFESP Google Scholar
Furtado Naves, Zita Valeria Google Scholar
Ibelli Vaz, Tania Mara Google Scholar
Irino, Kinue Google Scholar
Instituição Inst Adolfo Lutz Registro
Univ Fed Juiz de Fora
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Federal de São Paulo (UNIFESP)
Resumo The prevalence, serotypes and virulence profiles of Shiga toxin-producing Escherichia coli (STEC) were investigated in 205 healthy beef and dairy cattle. and 106 goats reared in the southeastern region of Minas Gerais State, Brazil. the prevalence of STEC was 57.5% (61/106) in goats, 39.2%, (40/102) in beef cattle and 17.5% (18/103) in dairy cattle. Among the 514 STEC isolates, 40 different serotypes were found and some of them were identified in a specific host. STEC isolates harboring stx(1) corresponded to 15.6% (28/180), 26.7% (16/60) and 24.1% (661274) in beef cattle, dairy cattle and goats. respectively. Stx(2) was found in 30% (54/180), 53.3% (32/60) and 34.7% (95/274) of beef and dairy cattle, and goats. stx(1) Plus Stx(2) sequences were harbored by 54.4% (98/180), 20% (12/60) and 41.2% (113/274) of beef cattle, dairy cattle and goats. respectively. the eae sequence was found in 15% (9/60) and 0.6% (1/180) of STEC isolates from dairy and beef cattle, respectively, and the toxB gene was found only in one O157:H7 strain isolated from beef cattle. Strains with the genetic profiles stx(2) ehxA iha saa and stx(1) stx(2) ehxA iha saa were the most prevalent among STEC isolates from cattle. Profiles stx, Stx(2) ehxA ilia, stx2, and stx, iha accounted for 75.5% (207 /274) of the STEC isolates from goats. While STEC strains carrying either Stx(2) alone or associated with stx(1) were found more frequently in cattle, those harboring sequences stx(1), and Stx(2d) alone or associated with stx(1c) predominated in goats. Our data show a diversity of STEC strains in food-producing animals, most of them carrying genes linked to severe forms of human diseases. (C) 2008 Elsevier B.V. All rights reserved.
Palavra-chave Escherichia coli
Shiga toxin
STEC serotypes
O157 : H7
genotypes
virulence markers
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Número do financiamento FAPESP: 03/12193-6
Data de publicação 2008-09-30
Publicado em International Journal of Food Microbiology. Amsterdam: Elsevier B.V., v. 127, n. 1-2, p. 139-146, 2008.
ISSN 0168-1605 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 139-146
Fonte http://dx.doi.org/10.1016/j.ijfoodmicro.2008.06.023
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000259771300021
Endereço permanente http://repositorio.unifesp.br/handle/11600/30912

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta