Effect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats

Effect of ultrasound therapy on the repair of Gastrocnemius muscle injury in rats

Autor Balejo Piedade, Maria Cristina Autor UNIFESP Google Scholar
Galhardo, Milene Sanches Autor UNIFESP Google Scholar
Battlehner, Claudia Naves Google Scholar
Ferreira, Marcelo Alves Google Scholar
Caldini, Elia Garcia Google Scholar
Szymanski de Toledo, Olga Maria Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo The aim of this study was to evaluate the effect of the pulsed ultrasound therapy (PUT) in stimulating myoregeneration and collagen deposition in an experimental model of lacerative gastrocnemius muscle lesion in 30 Wistar rats. Fifteen rats were treated (TG) daily with 1 MHz pulsed ultrasound (50%) at 0.57 W/cm(2) for 5 min, and 15 were control animals (CG). Muscle samples were analyzed on postoperative days 4, 7 and 14 through H&E, Picrosirius-polarization and immunohistochemistry for desmin. the lesions presented similar inflammatory responses in both treated and control groups. the areal fraction of fibrillar collagen was larger in the TG at 4 days post-operatively (17.53 +/- 6.2% vs 6.79 +/- 1.3%, p = 0.0491), 7 days (31.07 +/- 7.45% vs 12.57 +/- 3.6%, p = 0.0021) and 14 days (30.39 +/- 7.3% vs 19.13 +/- 3.51%, p = 0.0118); the areal fraction of myoblasts and myotubes was larger in the TG at 14 days after surgery (41.66 +/- 2.97% vs 34.83 +/- 3.08%, p = 0.025). Our data suggest that the PUT increases the differentiation of muscular lineage cells, what would favor tissue regeneration. On the other hand, it is also suggested that there is a larger deposition of collagenous fibers, what could mean worse functional performance. However, the percentage of fibers seems to have stabilized at day 7 in TG and kept increasing in CG. Furthermore, the collagen supramolecular organization achieved by the TG is also significant according to the Sirius red staining results. (C) 2008 Elsevier B.V. All rights reserved.
Palavra-chave myoregeneration
fibrosis
collagen
myotubes
desmin
morphometry
Idioma Inglês
Data de publicação 2008-09-01
Publicado em Ultrasonics. Amsterdam: Elsevier B.V., v. 48, n. 5, p. 403-411, 2008.
ISSN 0041-624X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 403-411
Fonte http://dx.doi.org/10.1016/j.ultras.2008.01.009
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258759000008
Endereço permanente http://repositorio.unifesp.br/handle/11600/30889

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