Lesions of medullary catecholaminergic neurons increase salt intake in rats

Lesions of medullary catecholaminergic neurons increase salt intake in rats

Autor Almeida Colombari, Debora Simoes Autor UNIFESP Google Scholar
Pedrino, Gustavo Rodrigues Autor UNIFESP Google Scholar
Freiria-Oliveira, Andre Henrique Autor UNIFESP Google Scholar
Korim, Willian Seiji Autor UNIFESP Google Scholar
Maurino, Isabela Coutinho Autor UNIFESP Google Scholar
Cravo, Sergio Luiz Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Several findings suggest that catecholaminergic neurons in the caudal ventrolateral medulla (CVLM) contribute to body fluid homeostasis and cardiovascular regulation. From the CVLM other areas in central nervous system involved in cardiovascular regulation and hydroelectrolyte balance can be activated. Therefore, the aim of the present study was to investigate the effects of lesions of these neurons on 0.3 M NaCl and water intake induced by subcutaneous injection of furosemide (FURO) + captopril (CAP) or 36h of water deprivation/partial hydration with only water (WD/PR). Male Wistar rats (320-360g) were submitted to medullary catecholaminergic neuron lesions by microinjection of antidopamine-beta-hydroxylase-saporin (anti-DOH-saporin; 6.3ng in 60nl) into the CVLM (SAP-rats). Sham rats received microinjections of free saporin (1.3 ng in 60 nl) in the same region. in SAP-rats, the 0.3 M NaCl intake was increased after FURO+CAP (6.8 +/- 1.0m]/2h, vs. sham: 33 03ml/2h) as well as after WD/PR (11.1 +/- 1.3 ml/2 h vs. sham: 6.1 +/- 1.8 ml/2 h). Conversely, in SAP-rats, the water intake induced by FURO + CAP (14.8 +/- 1.3 ml/2 h, vs. sham: 14.1 +/- 1.6 ml/2 h) or by WD/PR (3.6 +/- 0.9 ml/2 h, vs. sham: 3.2 +/- 1.1 ml/2 h) was not different from sham rats. Immunohistochemical analysis indicates that microinjections of anti-D beta H-saporin produced extensive destruction within the A1 cell groups in the CVLM. These results suggest an inhibitory role for medullary catecholaminergic neurons on sodium appetite. (c) 2008 Elsevier Inc. All rights reserved.
Palavra-chave salt appetite
electrolyte balance
A1
CVLM
angiotensin II
noradrenaline
Idioma Inglês
Data de publicação 2008-08-15
Publicado em Brain Research Bulletin. Oxford: Pergamon-Elsevier B.V., v. 76, n. 6, p. 572-578, 2008.
ISSN 0361-9230 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 572-578
Fonte http://dx.doi.org/10.1016/j.brainresbull.2008.04.001
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258057600004
Endereço permanente http://repositorio.unifesp.br/handle/11600/30853

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