rBmTI-6, a Kunitz-BPTI domain protease inhibitor from the tick Boophilus microplus, its cloning, expression and biochemical characterization

rBmTI-6, a Kunitz-BPTI domain protease inhibitor from the tick Boophilus microplus, its cloning, expression and biochemical characterization

Author Sasaki, Sergio D. Google Scholar
Tanaka, Aparecida S. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
Abstract Boophilus microplus is a rich source of trypsin inhibitors, numerous Kunitz-BPTI (bovine pancreatic trypsin inhibitor) inhibitors have been described from larvae and eggs, named BmTIs. Among them, were characterized inhibitors for trypsin, human neutrophil elastase, human plasma kallikrein and plasmin. BmTIs elicited a protective immunological response against B. microplus infestation in cattle. However, only a small amount of purified natural BmTIs can be obtained from larvae and eggs by chromatographic methods, thus if BmTIs are to be used as vaccine antigens (immunogens) the production of recombinant BmTIs (rBmTIs) is essential. in this work we describe the cloning, expression, purification and characterization of rBmTI-6. rBmTI-6 is a three-headed Kunitz-BPTI inhibitor, expressed in the Pichia pastoris system. Although rBmTI-6 was processed by proteases and glycosylated during the expression process, these post-translational modifications did not alter the ability of rBmTI-6 to inhibit protease activity. Purified rBmTI-6 inhibited trypsin and plasmin. (C) 2008 Elsevier B.V. All rights reserved.
Keywords tick
Boophilus microplus
protease inhibitor
trypsin inhibitor
Kunitz
Language English
Date 2008-08-01
Published in Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 155, n. 1-2, p. 133-141, 2008.
ISSN 0304-4017 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 133-141
Origin http://dx.doi.org/10.1016/j.vetpar.2008.03.031
Access rights Closed access
Type Article
Web of Science ID WOS:000257980600018
URI http://repositorio.unifesp.br/handle/11600/30825

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