Hyaluronan involvement in the changes of mouse interpubic tissue during late pregnancy and post-partum

Hyaluronan involvement in the changes of mouse interpubic tissue during late pregnancy and post-partum

Autor Garcia, Eduardo A. Google Scholar
Veridiano, Adriano M. Google Scholar
Martins, Joao R. M. Autor UNIFESP Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Pinheiro, M. C. Autor UNIFESP Google Scholar
Joazeiro, Paulo P. Google Scholar
Toledo, Olga M. S. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Resumo The present work quantifies hyaluronan (HA) during the late pregnancy and post-partum in order to provide a better understanding of the role of HA in the adaptations that occur in the pubic symphysis during this period. HA was quantified in situ (histochemically) and in interpubic tissue extracts by fluorimetric assay. Samples were taken from virgin mice and from pregnant animals at various stages of pregnancy: 12th- 18th days into pregnancy, the day of delivery (D19) and the 3rd and 5th day post-partum. the quantitative fluorimetric analysis indicated a gradual increase of HA in the interpubic tissue throughout late pregnancy (2.4-4.6 mu g/mg dry weight). This was followed by a decrease beginning on D19 (12.4 mu g/mg), reaching close to virgin levels (2.2 mu g/mg) on the 5th day post-partum. the same optical density changes could be seen in the HA staining. Furthermore, the histochemical analysis demonstrated the presence of HA both in the extracellular matrix of the tissue and within its cells. Such results indicate that the extracellular presence of HA may contribute to the transformation of the symphysis into a flexible structure. in addition, HA's intracellular presence (until the 18th day of pregnancy) may contribute to cellular proliferation. Finally, during parturition and on the 5th day post-partum, HA may contribute to the maintenance of the myofibroblastic phenotype of ligament cells, aiding the ligament involution after parturition. (c) 2008 International Federation for Cell Biology. Published by Elsevier B.V. All rights reserved.
Palavra-chave Hyaluronan
Pubic symphysis
Histochemistry
HA-biotinylated probe
Pregnancy
Mice
HA-Elisa-like assay
Idioma Inglês
Financiador Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2008-08-01
Publicado em Cell Biology International. London: Academic Press Ltd Elsevier B.V., v. 32, n. 8, p. 913-919, 2008.
ISSN 1065-6995 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 913-919
Fonte http://dx.doi.org/10.1016/j.cellbi.2008.04.006
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000260671600007
Endereço permanente http://repositorio.unifesp.br/handle/11600/30820

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