alpha(1)-adrenoceptors trigger the snake venom production cycle in secretory cells by activating phosphatidylinositol 4,5-bisphosphate hydrolysis and ERK signaling pathway

alpha(1)-adrenoceptors trigger the snake venom production cycle in secretory cells by activating phosphatidylinositol 4,5-bisphosphate hydrolysis and ERK signaling pathway

Autor Kerchove, Celine M. Google Scholar
Luna, Milene S. A. Google Scholar
Zablith, Mariana B. Google Scholar
Lazari, Maria F. M. Autor UNIFESP Google Scholar
Smaili, Soraya S. Autor UNIFESP Google Scholar
Yamanouye, Norma Google Scholar
Instituição Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Resumo Loss of venom from the venom gland after biting or manual extraction leads to morphological changes in venom secreting cells and the start of a cycle of production of new venom. We have previously shown that stimulation of both (alpha- and beta-adrenoceptors in the secretory cells of the venom gland is essential for the onset of the venom production cycle in Bothrops jararaca. We investigated the signaling pathway by which the alpha(1)-adrenoceptor initiates the venom production cycle. Our results show that the alpha(1)-adrenoceptor subtype is present in venom gland of the snake. in quiescent cells, stimulation of alpha(1)-adrenoceptor with phenylephrine increased the total inositol phosphate concentration, and this effect was blocked by the phospholipase C inhibitor U73122. Phenylephrine mobilized Ca(2+) from thapsigargin-sensitive stores and increased protein kinase C activity. in addition, alpha(1)-adrenoceptor stimulation increased the activity of ERK 1/2, partially via protein kinase C. Using RTPCR approach we obtained a partial sequence of a snake alpha(1)-adrenoceptor (260 bp) with higher identity with alpha(1)- and alpha(1B)-adrenoceptor from different species. These results suggest that alpha(1)-adrenoceptor in the venom secreting cells are probably coupled to a G(q) protein and trigger the venom production cycle by activating the phosphatidylinositol 4,5-bisphosphate and ERK signaling pathway. (c) 2008 Elsevier Inc. All rights reserved.
Palavra-chave alpha(1)-adrenoceptor
ERK
partial sequence
PIP(2) hydrolysis
snake
venom gland
Idioma Inglês
Data de publicação 2008-08-01
Publicado em Comparative Biochemistry and Physiology A-molecular & Integrative Physiology. New York: Elsevier B.V., v. 150, n. 4, p. 431-437, 2008.
ISSN 1095-6433 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 431-437
Fonte http://dx.doi.org/10.1016/j.cbpa.2008.04.607
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258056100009
Endereço permanente http://repositorio.unifesp.br/handle/11600/30819

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