Novel protective antigens expressed by Trypanosoma cruzi amastigotes provide immunity to mice highly susceptible to Chagas' disease

Novel protective antigens expressed by Trypanosoma cruzi amastigotes provide immunity to mice highly susceptible to Chagas' disease

Autor Silveira, Eduardo L. V. Autor UNIFESP Google Scholar
Claser, Carla Autor UNIFESP Google Scholar
Haolla, Filipe A. B. Autor UNIFESP Google Scholar
Zanella, Luiz G. Autor UNIFESP Google Scholar
Rodrigues, Mauricio M. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. in our experiments, we used this mouse model to search for other amastigote-expressed ORFs with a similar property. Fourteen ORFs previously determined to be expressed in this developmental stage were individually inserted into a eukaryotic expression vector containing a nucleotide sequence that encoded a mammalian secretory signal peptide. Immunization with 13 of the 14 ORFs induced specific antibodies which recognized the amastigotes. Three of those immune sera also reacted with trypomastigotes and epimastigotes. After a lethal challenge with Y strain trypomastigotes, the vast majority of plasmid-injected mice succumbed to infection. in some cases, a significant delay in mortality was observed. Only two of these ORFs provided protective immunity against the otherwise lethal infection caused by trypomastigotes of the Y or Colombia strain. These ORFs encode members of the trans-sialidase family of surface antigens related to the previously described protective antigen amastigote surface protein 2 (ASP-2). Nevertheless, at the level of antibody recognition, no cross-reactivity was observed between the ORFs and the previously described ASP-2 from the Y strain. in immunofluorescence analyses, we observed the presence of epitopes related to both proteins expressed by amastigotes of seven different strains. in conclusion, our approach allowed us to successfully identify two novel protective ORFs which we consider interesting for future studies on the immune response to Chagas' disease.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Millennium Institute for Vaccine Development and Technology
Millennium Institute for Gene Therapy
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento Millennium Institute for Vaccine Development and Technology: CNPq-420067/2005-1
Data de publicação 2008-08-01
Publicado em Clinical and Vaccine Immunology. Washington: Amer Soc Microbiology, v. 15, n. 8, p. 1292-1300, 2008.
ISSN 1556-6811 (Sherpa/Romeo, fator de impacto)
Publicador Amer Soc Microbiology
Extensão 1292-1300
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000258667200022
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