Humoral immune response after genetic immunization is consistently improved by electroporation

Humoral immune response after genetic immunization is consistently improved by electroporation

Autor Parise, Carolina Bellini Autor UNIFESP Google Scholar
Lisboa, Bianca Autor UNIFESP Google Scholar
Takeshita, Daniela Autor UNIFESP Google Scholar
Sacramento, Chester Bittencourt Autor UNIFESP Google Scholar
Moraes, Jane Zveiter de Autor UNIFESP Google Scholar
Han, Sang Won Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Aiming to evaluate some parameters to influence the immune response to DNA vaccination, we compare three protocols of DNA immunization (i.m. injections, i.m. injections followed by electroporation, and the effect of i.p. injection of stably antigen-transfected cells before DNA administration), using three different antigens. Statistical analyses showed that electroporation after intramuscular injections provided an immune response comparable to that obtained by pre-treatment with antigen-transfected cells and similar to that obtained by protein immunization. the results allowed us selecting a protocol that worked well for all three antigens and reinforced the idea that high level of gene expression is essential to get good immunization. (c) 2008 Elsevier B.V. All rights reserved.
Palavra-chave DNA vaccine
genetic immunization
electroporation
vaccine
Idioma Inglês
Data de publicação 2008-07-23
Publicado em Vaccine. Oxford: Elsevier B.V., v. 26, n. 31, p. 3812-3817, 2008.
ISSN 0264-410X (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 3812-3817
Fonte http://dx.doi.org/10.1016/j.vaccine.2008.05.029
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258009000005
Endereço permanente http://repositorio.unifesp.br/handle/11600/30804

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