Short communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cells

Short communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cells

Author Guimaraes, Ana Paula Autor UNIFESP Google Scholar
Sa-Filho, Dercy Jose Autor UNIFESP Google Scholar
Sucupira, Maria Cecilia Autor UNIFESP Google Scholar
Janini, Luiz Mario Autor UNIFESP Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Genotypic resistance is currently assessed through direct sequencing, which cannot detect resistant strains below 20%. We compared the genotypic resistance profile of virions and proviruses using population-based analysis and single genome sequencing of the protease region of the pol gene in samples collected from five individuals in whom indinavir monotherapy resulted in treatment failure. Single genome sequencing showed that not all strains present the same resistance mutations, which can be dispersed across different HIV genomes. the resistance profile found in plasma was very similar to that found in peripheral blood mononuclear cells (PBMCs), confirming the utility of assessing proviral DNA as for a means of determining antiretroviral resistance.
Language English
Date 2008-07-01
Published in Aids Research and Human Retroviruses. New Rochelle: Mary Ann Liebert Inc, v. 24, n. 7, p. 967-971, 2008.
ISSN 0889-2229 (Sherpa/Romeo, impact factor)
Publisher Mary Ann Liebert Inc
Extent 967-971
Origin http://dx.doi.org/10.1089/aid.2008.0020
Access rights Closed access
Type Article
Web of Science ID WOS:000258302000011
URI http://repositorio.unifesp.br/handle/11600/30787

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