Short communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cells

Short communication - Profiling resistance-related mutations in the protease region of the pol gene: Single genome sequencing of HIV in plasma and peripheral blood mononuclear cells

Autor Guimaraes, Ana Paula Autor UNIFESP Google Scholar
Sa-Filho, Dercy Jose Autor UNIFESP Google Scholar
Sucupira, Maria Cecilia Autor UNIFESP Google Scholar
Janini, Luiz Mario Autor UNIFESP Google Scholar
Diaz, Ricardo Sobhie Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Genotypic resistance is currently assessed through direct sequencing, which cannot detect resistant strains below 20%. We compared the genotypic resistance profile of virions and proviruses using population-based analysis and single genome sequencing of the protease region of the pol gene in samples collected from five individuals in whom indinavir monotherapy resulted in treatment failure. Single genome sequencing showed that not all strains present the same resistance mutations, which can be dispersed across different HIV genomes. the resistance profile found in plasma was very similar to that found in peripheral blood mononuclear cells (PBMCs), confirming the utility of assessing proviral DNA as for a means of determining antiretroviral resistance.
Idioma Inglês
Data de publicação 2008-07-01
Publicado em Aids Research and Human Retroviruses. New Rochelle: Mary Ann Liebert Inc, v. 24, n. 7, p. 967-971, 2008.
ISSN 0889-2229 (Sherpa/Romeo, fator de impacto)
Publicador Mary Ann Liebert Inc
Extensão 967-971
Fonte http://dx.doi.org/10.1089/aid.2008.0020
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258302000011
Endereço permanente http://repositorio.unifesp.br/handle/11600/30787

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