Gene expression profile analysis of primary glioblastomas and non-neoplastic brain tissue: identification of potential target genes by oligonucleotide microarray and real-time quantitative PCR

Gene expression profile analysis of primary glioblastomas and non-neoplastic brain tissue: identification of potential target genes by oligonucleotide microarray and real-time quantitative PCR

Autor Scrideli, Carlos A. Google Scholar
Carlotti, Carlos G. Google Scholar
Okamoto, Oswaldo K. Autor UNIFESP Google Scholar
Andrade, Vanessa S. Google Scholar
Cortez, Maria A. A. Google Scholar
Motta, Fabio J. N. Google Scholar
Lucio-Eterovic, Agda K. Google Scholar
Neder, Luciano Google Scholar
Rosemberg, Sergio Google Scholar
Oba-Shinjo, Sueli M. Google Scholar
Marie, Suely K. N. Google Scholar
Tone, Luiz G. Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Resumo The prognosis of glioblastomas is still extremely poor and the discovery of novel molecular therapeutic targets can be important to optimize treatment strategies. Gene expression analyses comparing normal and neoplastic tissues have been used to identify genes associated with tumorigenesis and potential therapeutic targets. We have used this approach to identify differentially expressed genes between primary glioblastomas and non-neoplastic brain tissues. We selected 20 overexpressed genes related to cell cycle, cellular movement and growth, proliferation and cell-to-cell signaling and analyzed their expression levels by real time quantitative PCR in cDNA obtained from microdissected fresh tumor tissue from 20 patients with primary glioblastomas and from 10 samples of non-neoplastic white matter tissue. the gene expression levels were significantly higher in glioblastomas than in non-neoplastic white matter in 18 out of 20 genes analyzed: P < 0.00001 for CDKN2C, CKS2, EEF1A1, EMP3, PDPN, BNIP2, CA12, CD34, CDC42EP4, PPIE, SNAI2, GDF15 and MMP23b; and NFIA (P: 0.0001), GPS1 (P: 0.0003), LAMA1 (P: 0.002), STIM1 (P: 0.006), and TASP1 (P: 0.01). Five of these genes are located in contiguous loci at 1p31-36 and 2 at 17q24-25 and 8 of them encode surface membrane proteins. PDPN and CD34 protein expression were evaluated by immunohistochemistry and they showed concordance with the PCR results. the present results indicate the presence of 18 overexpressed genes in human primary glioblastomas that may play a significant role in the pathogenesis of these tumors and that deserve further functional investigation as attractive candidates for new therapeutic targets.
Palavra-chave glioblastoma
brain
gene expression
microarray
RQ-PCR
Idioma Inglês
Data de publicação 2008-07-01
Publicado em Journal of Neuro-oncology. New York: Springer, v. 88, n. 3, p. 281-291, 2008.
ISSN 0167-594X (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 281-291
Fonte http://dx.doi.org/10.1007/s11060-008-9579-4
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000256339900005
Endereço permanente http://repositorio.unifesp.br/handle/11600/30757

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