Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle

Automated Nuclear Analysis of Leishmania major Telomeric Clusters Reveals Changes in Their Organization during the Parasite's Life Cycle

Autor Dossin, Fernando de M. Google Scholar
Dufour, Alexandre Google Scholar
Dusch, Elodie Google Scholar
Siqueira-Neto, Jair L. Google Scholar
Moraes, Carolina B. Autor UNIFESP Google Scholar
Yang, Gyong Seon Google Scholar
Cano, Maria Isabel Google Scholar
Genovesio, Auguste Google Scholar
Freitas-Junior, Lucio H. Google Scholar
Instituição Inst Pasteur Korea
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista
Resumo Parasite virulence genes are usually associated with telomeres. the clustering of the telomeres, together with their particular spatial distribution in the nucleus of human parasites such as Plasmodium falciparum and Trypanosoma brucei, has been suggested to play a role in facilitating ectopic recombination and in the emergence of new antigenic variants. Leishmania parasites, as well as other trypanosomes, have unusual gene expression characteristics, such as polycistronic and constitutive transcription of protein-coding genes. Leishmania subtelomeric regions are even more unique because unlike these regions in other trypanosomes they are devoid of virulence genes. Given these peculiarities of Leishmania, we sought to investigate how telomeres are organized in the nucleus of Leishmania major parasites at both the human and insect stages of their life cycle. We developed a new automated and precise method for identifying telomere position in the three-dimensional space of the nucleus, and we found that the telomeres are organized in clusters present in similar numbers in both the human and insect stages. While the number of clusters remained the same, their distribution differed between the two stages. the telomeric clusters were found more concentrated near the center of the nucleus in the human stage than in the insect stage suggesting reorganization during the parasite's differentiation process between the two hosts. These data provide the first 3D analysis of Leishmania telomere organization. the possible biological implications of these findings are discussed.
Idioma Inglês
Financiador Institut Pasteur Korea
Data 2008-06-11
Publicado em Plos One. San Francisco: Public Library Science, v. 3, n. 6, 6 p., 2008.
ISSN 1932-6203 (Sherpa/Romeo, fator de impacto)
Editor Public Library Science
Extensão 6
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000263248800003

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