Photodynamic therapy for root canals infected with Enterococcus faecalis

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dc.contributor.author Fonseca, Michelle Bernardes
dc.contributor.author Tessare, Paulo Odair
dc.contributor.author Pallota, Raul Capp
dc.contributor.author Ferreira Filho, Hely
dc.contributor.author Porto Denardin, Odilon Victor [UNIFESP]
dc.contributor.author Rapoport, Abrao
dc.contributor.author Dedivitis, Rogerio Aparecido [UNIFESP]
dc.contributor.author Veronezi, Joao Francisco
dc.contributor.author Genovese, Walter Joao
dc.contributor.author Franco Ricardo, Ana Lucia
dc.date.accessioned 2016-01-24T13:51:27Z
dc.date.available 2016-01-24T13:51:27Z
dc.date.issued 2008-06-01
dc.identifier http://dx.doi.org/10.1089/pho.2007.2124
dc.identifier.citation Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 26, n. 3, p. 209-213, 2008.
dc.identifier.issn 1549-5418
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/30715
dc.description.abstract Objective: the aim of this study was to investigate the effects of photodynamic therapy (PDT) on endodontic pathogens by evaluating the decrease in numbers of Enterococcus faecalis colonies in the canals of extracted human teeth.Background Data: Failure in endodontics is usually related to inadequate cleaning and disinfection of the root canal system. This is due to the establishment of microorganisms in areas where the instruments and chemical agents used during root canal preparation cannot eliminate them. PDT is a complementary therapeutic method that could be used to eliminate these remaining bacteria. PDT is a process in which radiation acts on a dye that is applied to the target organism, resulting in bacterial death.Materials and Methods: Forty-six uniradicular teeth had their canals contaminated with bacteria and were incubated for 48 h at 35 degrees C. After that, the teeth were divided into a control group (CG) and a test group (TG). the 23 CG teeth did not undergo any intervention, whereas in the TG the teeth received a solution of 0.0125% toluidine blue for 5 min followed by irradiation using a 50-mW diode laser (Ga-Al-As) at a wavelength of 660 nm. Bacterial samples were taken before and after irradiation. in each of the samples, the number of colony-forming units (CFU) was counted.Results: the mean decrease in CFU was 99.9% in the TG, whereas in the CG an increase of 2.6% was observed.Conclusion: PDT was effective as a bactericidal agent in Enterococcus faecalis-contaminated root canals. en
dc.format.extent 209-213
dc.language.iso eng
dc.publisher Mary Ann Liebert Inc
dc.relation.ispartof Photomedicine and Laser Surgery
dc.rights Acesso restrito
dc.title Photodynamic therapy for root canals infected with Enterococcus faecalis en
dc.type Artigo
dc.contributor.institution Hosp Heliopolis
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Hosp CEMA
dc.contributor.institution Sao Leopoldo Mand Univ
dc.description.affiliation Hosp Heliopolis, Clin Pathol Lab, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Dent, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Med, Dept Surg, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Otorhinolaryngol Head & Neck Surg, São Paulo, Brazil
dc.description.affiliation Hosp CEMA, Head Adm, São Paulo, Brazil
dc.description.affiliation Sao Leopoldo Mand Univ, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Otorhinolaryngol Head & Neck Surg, São Paulo, Brazil
dc.identifier.doi 10.1089/pho.2007.2124
dc.description.source Web of Science
dc.identifier.wos WOS:000257718400006



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