Photodynamic therapy for root canals infected with Enterococcus faecalis

Photodynamic therapy for root canals infected with Enterococcus faecalis

Autor Fonseca, Michelle Bernardes Google Scholar
Tessare, Paulo Odair Google Scholar
Pallota, Raul Capp Google Scholar
Ferreira Filho, Hely Google Scholar
Porto Denardin, Odilon Victor Autor UNIFESP Google Scholar
Rapoport, Abrao Google Scholar
Dedivitis, Rogerio Aparecido Autor UNIFESP Google Scholar
Veronezi, Joao Francisco Google Scholar
Genovese, Walter Joao Google Scholar
Franco Ricardo, Ana Lucia Google Scholar
Instituição Hosp Heliopolis
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Hosp CEMA
Sao Leopoldo Mand Univ
Resumo Objective: the aim of this study was to investigate the effects of photodynamic therapy (PDT) on endodontic pathogens by evaluating the decrease in numbers of Enterococcus faecalis colonies in the canals of extracted human teeth.Background Data: Failure in endodontics is usually related to inadequate cleaning and disinfection of the root canal system. This is due to the establishment of microorganisms in areas where the instruments and chemical agents used during root canal preparation cannot eliminate them. PDT is a complementary therapeutic method that could be used to eliminate these remaining bacteria. PDT is a process in which radiation acts on a dye that is applied to the target organism, resulting in bacterial death.Materials and Methods: Forty-six uniradicular teeth had their canals contaminated with bacteria and were incubated for 48 h at 35 degrees C. After that, the teeth were divided into a control group (CG) and a test group (TG). the 23 CG teeth did not undergo any intervention, whereas in the TG the teeth received a solution of 0.0125% toluidine blue for 5 min followed by irradiation using a 50-mW diode laser (Ga-Al-As) at a wavelength of 660 nm. Bacterial samples were taken before and after irradiation. in each of the samples, the number of colony-forming units (CFU) was counted.Results: the mean decrease in CFU was 99.9% in the TG, whereas in the CG an increase of 2.6% was observed.Conclusion: PDT was effective as a bactericidal agent in Enterococcus faecalis-contaminated root canals.
Idioma Inglês
Data de publicação 2008-06-01
Publicado em Photomedicine and Laser Surgery. New Rochelle: Mary Ann Liebert Inc, v. 26, n. 3, p. 209-213, 2008.
ISSN 1549-5418 (Sherpa/Romeo, fator de impacto)
Publicador Mary Ann Liebert Inc
Extensão 209-213
Fonte http://dx.doi.org/10.1089/pho.2007.2124
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000257718400006
Endereço permanente http://repositorio.unifesp.br/handle/11600/30715

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