Sertoli cell morphological alterations in albino rats treated with etoposide during prepubertal phase

Sertoli cell morphological alterations in albino rats treated with etoposide during prepubertal phase

Author Stumpp, Taiza Autor UNIFESP Google Scholar
Freymuller, Edna Autor UNIFESP Google Scholar
Miraglia, Sandra M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Sertoli cells are very important to spermatogenesis homeostasis because they control germ cell proliferation, differentiation, and death. Damages to Sertoli cells cause germ cell death and affect fertility. Etoposide is a potent chemotherapeutic drug largely used against a variety of cancers. However, this drug also kills normal cells, especially those undergoing rapid proliferation. in the testis, etoposide acts predominantly on intermediate and type B spermatogonia. Etoposide was shown to permanently alter Sertoli cell function when administered to prepubertal rats. Based on this, we decided to investigate whether etoposide can affect Sertoli cell morphology. for this, 25-day-old rats were treated with etoposide during 8 consecutive days and killed at 32, 45, 64, 127, and 180 days old. Testes were fixed in Bonin's liquid or in a mixture of 2.5% glutaraldehyde and 2% formaldehyde for analysis under light and electron microscopes, respectively. Sertoli cells showed morphological alterations such as the presence of chromatin clumps close to the nuclear membrane, nucleus displacement, and cytoplasmic vacuolization. Some Sertoli cells also showed nuclear and cytoplasmic degenerative characteristics, suggesting that etoposide causes severe damages to Sertoli cell.
Keywords testis
Sertoli cell
etoposide
spermatogenesis
electron microscopy
Language English
Date 2008-06-01
Published in Microscopy and Microanalysis. New York: Cambridge Univ Press, v. 14, n. 3, p. 225-235, 2008.
ISSN 1431-9276 (Sherpa/Romeo, impact factor)
Publisher Cambridge Univ Press
Extent 225-235
Origin http://dx.doi.org/10.1017/S1431927608080318
Access rights Closed access
Type Article
Web of Science ID WOS:000256377400002
URI http://repositorio.unifesp.br/handle/11600/30710

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