Sertoli cell morphological alterations in albino rats treated with etoposide during prepubertal phase

Sertoli cell morphological alterations in albino rats treated with etoposide during prepubertal phase

Autor Stumpp, Taiza Autor UNIFESP Google Scholar
Freymuller, Edna Autor UNIFESP Google Scholar
Miraglia, Sandra M. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Sertoli cells are very important to spermatogenesis homeostasis because they control germ cell proliferation, differentiation, and death. Damages to Sertoli cells cause germ cell death and affect fertility. Etoposide is a potent chemotherapeutic drug largely used against a variety of cancers. However, this drug also kills normal cells, especially those undergoing rapid proliferation. in the testis, etoposide acts predominantly on intermediate and type B spermatogonia. Etoposide was shown to permanently alter Sertoli cell function when administered to prepubertal rats. Based on this, we decided to investigate whether etoposide can affect Sertoli cell morphology. for this, 25-day-old rats were treated with etoposide during 8 consecutive days and killed at 32, 45, 64, 127, and 180 days old. Testes were fixed in Bonin's liquid or in a mixture of 2.5% glutaraldehyde and 2% formaldehyde for analysis under light and electron microscopes, respectively. Sertoli cells showed morphological alterations such as the presence of chromatin clumps close to the nuclear membrane, nucleus displacement, and cytoplasmic vacuolization. Some Sertoli cells also showed nuclear and cytoplasmic degenerative characteristics, suggesting that etoposide causes severe damages to Sertoli cell.
Palavra-chave testis
Sertoli cell
etoposide
spermatogenesis
electron microscopy
Idioma Inglês
Data de publicação 2008-06-01
Publicado em Microscopy and Microanalysis. New York: Cambridge Univ Press, v. 14, n. 3, p. 225-235, 2008.
ISSN 1431-9276 (Sherpa/Romeo, fator de impacto)
Publicador Cambridge Univ Press
Extensão 225-235
Fonte http://dx.doi.org/10.1017/S1431927608080318
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000256377400002
Endereço permanente http://repositorio.unifesp.br/handle/11600/30710

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