B-1 cells facilitate Paracoccidioides brasiliensis infection in mice via IL-10 secretion

B-1 cells facilitate Paracoccidioides brasiliensis infection in mice via IL-10 secretion

Autor Popi, Ana Flavia Autor UNIFESP Google Scholar
Godoy, Luiz Claudio Autor UNIFESP Google Scholar
Xander, Patricia Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ Massachusetts
Resumo Protective immunity in paracoccidioidomycosis is mainly mediated by cellular immunity. the role of B cells in this disease, in particular B-1 cells, is poorly understood. the aim of this study was to characterize the participation of B-1 cells in resistance or susceptibility of BALB/c and BALB/Xid mice to P. brasiliensis (Pb) pulmonary infection. BALB/Xid, which lacks B-1 cells, exhibited higher resistance to infection when compared with BALB/c mice. However, adoptive transfer of B-1 cells to BALB/Xid mice drastically increased the susceptibility of these animals to Pb infection. the fungal burden in BALB/c and B-1 -reconstituted BALB/Xid was significantly higher as compared to BALB/Xid strain. Compact, well-organized granulomas were observed in the lungs of BALB/Xid mice, whereas large lesions with necrotic center with a plethora of fungi developed in BALB/c mice. It was also shown that B-1 cells impair phagocytosis of Pb by macrophages in vitro via secretion of IL-10, which was increased upon stimulation with a purified Pb antigen, gp43. Finally, in vivo blockade of IL-10 led to a better control of infection by the highly susceptible B10.A mouse. These findings suggest that B-1 cells play a major role in resistance/susceptibility to Pb infection in murine models, most likely via production of IL-10. (C) 2008 Elsevier Masson SAS. All rights reserved.
Assunto B-1 cells
Paracoccidioides brasiliensis
Idioma Inglês
Data 2008-06-01
Publicado em Microbes and Infection. Amsterdam: Elsevier B.V., v. 10, n. 7, p. 817-824, 2008.
ISSN 1286-4579 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 817-824
Fonte http://dx.doi.org/10.1016/j.micinf.2008.04.012
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000258013500014
URI http://repositorio.unifesp.br/handle/11600/30703

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