A proposed EPR approach to evaluating agonist binding site of a peptide receptor

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dc.contributor.author Lopes, Douglas D. [UNIFESP]
dc.contributor.author Poletti, Erick F. [UNIFESP]
dc.contributor.author Vieira, Renata F. F. [UNIFESP]
dc.contributor.author Jubilut, Guita N. [UNIFESP]
dc.contributor.author Oliveira, Laerte [UNIFESP]
dc.contributor.author Paiva, Antonio C. M. [UNIFESP]
dc.contributor.author Schreier, Shirley
dc.contributor.author Nakaie, Clovis R. [UNIFESP]
dc.date.accessioned 2016-01-24T13:51:25Z
dc.date.available 2016-01-24T13:51:25Z
dc.date.issued 2008-06-01
dc.identifier http://dx.doi.org/10.1007/s10989-007-9120-1
dc.identifier.citation International Journal of Peptide Research and Therapeutics. New York: Springer, v. 14, n. 2, p. 121-126, 2008.
dc.identifier.issn 1573-3149
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/30692
dc.description.abstract Angiotensin II (Ang II) and its transmembrane AT(1) receptor were selected in order to test an innovative strategy that might allow the assessment of the agonist binding site in the receptor molecule. With the use of the 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) paramagnetic probe, a biologically active agonist (TOAC(1)-Ang II), as well as an inactive control (TOAC(4)-Ang II) analogs were mixed in solution with various synthesized AT(1) fragments. Comparative intermolecular interactions, as estimated by analyzing the EPR spectra of solutions, suggested the existence of an agonist binding site containing a sequence composed of portions of the N-terminal (13-17) and the third extracellular loop (266-278) fragments of the AT(1) molecule. Therefore, this combined EPR-TOAC approach shows promise as an alternative for use also in other applications related to specific intermolecular association processes. en
dc.format.extent 121-126
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof International Journal of Peptide Research and Therapeutics
dc.rights Acesso restrito
dc.subject peptide en
dc.subject angiotensin II en
dc.subject receptor en
dc.subject TOAC en
dc.subject EPR en
dc.subject spin label en
dc.title A proposed EPR approach to evaluating agonist binding site of a peptide receptor en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Inst Chem, Dept Biochem, BR-05513870 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.identifier.doi 10.1007/s10989-007-9120-1
dc.description.source Web of Science
dc.identifier.wos WOS:000255878700007



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