A proposed EPR approach to evaluating agonist binding site of a peptide receptor

A proposed EPR approach to evaluating agonist binding site of a peptide receptor

Autor Lopes, Douglas D. Autor UNIFESP Google Scholar
Poletti, Erick F. Autor UNIFESP Google Scholar
Vieira, Renata F. F. Autor UNIFESP Google Scholar
Jubilut, Guita N. Autor UNIFESP Google Scholar
Oliveira, Laerte Autor UNIFESP Google Scholar
Paiva, Antonio C. M. Autor UNIFESP Google Scholar
Schreier, Shirley Google Scholar
Nakaie, Clovis R. Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Resumo Angiotensin II (Ang II) and its transmembrane AT(1) receptor were selected in order to test an innovative strategy that might allow the assessment of the agonist binding site in the receptor molecule. With the use of the 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (TOAC) paramagnetic probe, a biologically active agonist (TOAC(1)-Ang II), as well as an inactive control (TOAC(4)-Ang II) analogs were mixed in solution with various synthesized AT(1) fragments. Comparative intermolecular interactions, as estimated by analyzing the EPR spectra of solutions, suggested the existence of an agonist binding site containing a sequence composed of portions of the N-terminal (13-17) and the third extracellular loop (266-278) fragments of the AT(1) molecule. Therefore, this combined EPR-TOAC approach shows promise as an alternative for use also in other applications related to specific intermolecular association processes.
Palavra-chave peptide
angiotensin II
receptor
TOAC
EPR
spin label
Idioma Inglês
Data de publicação 2008-06-01
Publicado em International Journal of Peptide Research and Therapeutics. New York: Springer, v. 14, n. 2, p. 121-126, 2008.
ISSN 1573-3149 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 121-126
Fonte http://dx.doi.org/10.1007/s10989-007-9120-1
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000255878700007
Endereço permanente http://repositorio.unifesp.br/handle/11600/30692

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta