DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

DNA damage in lymphocytes and buccal mucosa cells of children with malignant tumours undergoing chemotherapy

Author Minicucci, Eliana Maria Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Camargo, Beatriz de Google Scholar
Costa, Maria C. Google Scholar
Ribeiro, Lucia Regina Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
AC Camargo Hosp
Abstract The aim of the present study was to evaluate DNA damage (micronucleus) in cytokinesis-blocked lymphocytes and exfoliated buccal mucosa cells from children with malignant tumours and under chemotherapy. Micronucleated cells (MNCs) were assessed from children before and during chemotherapy. A total of 21 healthy children (controls), matched for gender and age, were used as control. the results pointed out higher frequencies of micronucleated lymphocytes in children with malignant tumour before any therapy when compared to healthy probands. Furthermore an increase of micronucleated lymphocytes during chemotherapy was detected when compared to the data obtained before chemotherapy. No statistically significant increases of MNCs were noticed in buccal mucosa cells at any of the timepoints evaluated. Taken together, these data indicate that the presence of malignant tumours may increase the frequency of DNA damage in circulating lymphocytes, these cells being more sensitive for detecting chromosome aberrations caused by anti-cancer drugs.
Keywords Buccal mucosa cells
Chemotherapy
Cytokinesis-block micronucleus assay
Paediatric cancer
Language English
Date 2008-06-01
Published in Clinical and Experimental Medicine. New York: Springer, v. 8, n. 2, p. 79-85, 2008.
ISSN 1591-8890 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 79-85
Origin https://dx.doi.org/10.1007/s10238-008-0161-3
Access rights Closed access
Type Article
Web of Science ID WOS:000257022600004
URI https://repositorio.unifesp.br/handle/11600/30689

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