Immunolocalization of alpha(1A)-adrenoceptors in rat and human epididymis

Immunolocalization of alpha(1A)-adrenoceptors in rat and human epididymis

Autor Cava Queiroz, Daniel Barboza Autor UNIFESP Google Scholar
Porto, Catarina S. Autor UNIFESP Google Scholar
Grossman, Gail Google Scholar
Petrusz, Peter Google Scholar
W. Avellar, Maria Christina Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Univ N Carolina
Resumo Immunohistochemistry was conducted to analyze the cellular localization of alpha(1A)-adrenoceptors along rat and human epididymis. ADR-A, a polyclonal antibody that recognizes the specific C-terminal region of alpha(1A)-adrenoceptors, immunostained this adrenoceptor subtype in smooth muscle cells surrounding the epididymal tubules and interstitial blood vessels and in subpopulations of epithelial cells from adult rat and human caput and cauda epididymidis. the same cell types from rat epididymidis were immunostained by ADR-1, a polyclonal antibody that recognizes a common region of the three alpha(1)-adrenoceptor subtypes, alpha(1A), alpha(1B), and alpha(1D). Immunostaining with both antibodies was also conducted in adult rat and human vas deferens and seminal vesicle used as positive controls because of the abundance of alpha(1A)-adrenoceptors in these tissues. ADR-A- and ADR-1-positive immunostaining was differentially distributed depending on the antibody, method of tissue fixation (Bouin-fixed and fresh frozen tissues), species (rat and human), tissue (caput and cauda epididymidis), and age (immature and adult rats) analyzed. This is the first report immunolocalizing alpha(1A)-adrenoceptor along rat and human epididymis. the presence of this adrenoceptor subtype in epididymal smooth muscle and epithelial cells indicates their contribution to smooth muscle contractile responses and a possible role in the absorptive and/or secretory activities of the epithelium lining the epididymal duct. Taken together, our results should contribute to a better understanding of the physiological role of alpha(1)-adrenoceptors in the epididymidis and the importance of the sympathetic nervous system for male (in)fertility.
Assunto epididymidis
male reproductive tract
rat (Wistar)
Idioma Inglês
Data 2008-06-01
Publicado em Cell and Tissue Research. New York: Springer, v. 332, n. 3, p. 509-522, 2008.
ISSN 0302-766X (Sherpa/Romeo, fator de impacto)
Editor Springer
Extensão 509-522
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000256354500013

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