Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion

Bone marrow cells are able to increase vessels number during repair of sciatic nerve lesion

Author Fernandes, Marcela Autor UNIFESP Google Scholar
Valente, Sandra Gomes Autor UNIFESP Google Scholar
Silva Fernandes, Maria Jose da Autor UNIFESP Google Scholar
Villar Felix, Evandro Penteado Autor UNIFESP Google Scholar
Naffah Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Scerni, Debora Amado Autor UNIFESP Google Scholar
Gomes dos Santos, Joao Baptista Autor UNIFESP Google Scholar
Leite, Vilnei Mattioli Autor UNIFESP Google Scholar
Faloppa, Flavio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to compare the outcomes of nerve autografts (GRF) and venous grafts containing mononuclear bone marrow cells (BMCs) in sciatic nerve-lesioned rats. Control animals underwent sham operations (SHAM), received empty venous grafts (EPV), or received venous grafts containing BMC vehicle (AGR). Outcome was evaluated through sciatic functional index (SIFI), morphometric and morphologic analyses of the nerve distal to the lesion, and the number of spinal cord motor neurons positive for the retrograde tracer, Fluoro-Gold (R). All groups exhibited poor results in SFI when compared to SHAM animals throughout the postoperative period. All groups also had a significantly greater fiber density, decreased fiber diameter, and decreased motor neuron number than the SHAM group. No significant difference between the GRF and BMC groups was observed in any of these parameters. On the other hand, vessel density was significantly higher in BMC than all other groups. BMC-containing venous grafts are superior to nerve autografts in increasing vessel density during sciatic nerve regeneration. (c) 2007 Elsevier B.V. All rights reserved.
Keywords bone marrow cells
grafts
nerve regeneration
sciatic nerve
rats
Language English
Date 2008-05-15
Published in Journal of Neuroscience Methods. Amsterdam: Elsevier B.V., v. 170, n. 1, p. 16-24, 2008.
ISSN 0165-0270 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 16-24
Origin http://dx.doi.org/10.1016/j.jneumeth.2007.12.009
Access rights Closed access
Type Article
Web of Science ID WOS:000255697400003
URI http://repositorio.unifesp.br/handle/11600/30665

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